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Titolo:
Differences between rats and rabbits in NMDA receptor-mediated calcium signalling in hippocampal neurones
Autore:
Salinska, E; Ziembowicz, A; Gordon-Krajcer, W; Skangiel-Kramska, J; Jablonska, B; Makarewicz, D; Zieminska, E; Lazarewicz, JW;
Indirizzi:
Polish Acad Sci, Med Res Ctr, Dept Neurochem, PL-02106 Warsaw, Poland Polish Acad Sci Warsaw Poland PL-02106 eurochem, PL-02106 Warsaw, Poland Polish Acad Sci, M Nencki Inst Expt Biol, Warsaw, Poland Polish Acad Sci Warsaw Poland , M Nencki Inst Expt Biol, Warsaw, Poland
Titolo Testata:
BRAIN RESEARCH BULLETIN
fascicolo: 6, volume: 53, anno: 2000,
pagine: 813 - 819
SICI:
0361-9230(200012)53:6<813:DBRARI>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
CA2+ RELEASE CHANNELS; RYANODINE BINDING-SITES; IN-VIVO; BUFFERING PROPERTIES; GRANULE CELLS; BRAIN; PARVALBUMIN; ACTIVATION; EXPRESSION; CALRETININ;
Keywords:
calbindin D-28k; intracellular Ca2+; microdialysis; ryanodine receptors;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Lazarewicz, JW Polish Acad Sci, Med Res Ctr, Dept Neurochem, Pawinskiego 5, PL-02106 Warsaw, Poland Polish Acad Sci Pawinskiego 5 Warsaw Poland PL-02106 oland
Citazione:
E. Salinska et al., "Differences between rats and rabbits in NMDA receptor-mediated calcium signalling in hippocampal neurones", BRAIN RES B, 53(6), 2000, pp. 813-819

Abstract

In vivo microdialysis combined with the measurement of Ca-45(2+) efflux from prelabelled hippocampus demonstrated a pronounced N-methyl-D-aspartate (NMDA)-evoked Ca-45(2+) release to the dialysate in the rat dentate gyrus (DG) and CA1, whereas in rabbit a slight release of Ca-45(2+) was observed only in the DG. In vitro, we noticed that the NMDA-evoked increase in Fura-2 detected intracellular Ca2+ concentration in synaptoneurosomes from the rat, but not from the rabbit hippocampus, was strongly inhibited by the ryanodine receptor (RyR) antagonists dantrolene and ryanodine. To establish the mechanism of these differences, we characterised their possible dependence on the expression of RyR and their co-localisation with the calcium binding protein calbindin D-28k. A pronounced expression of [H-3]ryanodine binding sites in the rat DG, which is only slight in the CA1, was demonstrated whereas in rabbit they were only found in the DG, The pattern of expression of calbindin D-28k immunoreactivity and RyR in the rat and rabbit hippocampus was similar. These results suggest that the functional role of RyR in the generation of the NMDA receptor-mediated intracellular Ca2+ signalling in the rabbit hippocampal neurones is marginal when compared to the rat, These differences reflect a diverse expression of RyR in both species. The corresponding differences in calbindin D-28k immunoreactivity are most probably secondary in nature, (C) 2001 Elsevier Science Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 04:41:13