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Titolo:
Expression of type III sodium-dependent phosphate transporters/retroviral receptors mRNAs during osteoblast differentiation
Autore:
Nielsen, LB; Pedersen, FS; Pedersen, L;
Indirizzi:
Aarhus Univ, Dept Biol Mol & Struct, DK-8000 Aarhus C, Denmark Aarhus Univ Aarhus Denmark C iol Mol & Struct, DK-8000 Aarhus C, Denmark Aarhus Univ, Dept Med Microbiol & Immunol, DK-8000 Aarhus, Denmark Aarhus Univ Aarhus Denmark DK-8000 ol & Immunol, DK-8000 Aarhus, Denmark
Titolo Testata:
BONE
fascicolo: 2, volume: 28, anno: 2001,
pagine: 160 - 166
SICI:
8756-3282(200102)28:2<160:EOTISP>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
APE LEUKEMIA-VIRUS; TRANSPORTER RETROVIRUS RECEPTOR; CELL-SURFACE RECEPTORS; P-I TRANSPORT; MC3T3-E1 CELLS; ALKALINE-PHOSPHATASE; OSTEOGENIC CELLS; GENE-TRANSFER; BONE; PROTEIN;
Keywords:
phosphate-transport; Pit1; Pit2; MC3T3-E1; osteoblast; differentiation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Pedersen, L Aarhus Univ, Dept Biol Mol & Struct, Mollers Alle Bldg 130, DK-8000 AarhusC, Denmark Aarhus Univ Mollers Alle Bldg 130 Aarhus Denmark C C, Denmark
Citazione:
L.B. Nielsen et al., "Expression of type III sodium-dependent phosphate transporters/retroviral receptors mRNAs during osteoblast differentiation", BONE, 28(2), 2001, pp. 160-166

Abstract

Inorganic phosphate (Pi) is essential for the formation of bone. Pi transport in osteoblastic cells is mainly handled by sodium-dependent Pi (NaPi) transporters, different from the renal type I and II transporters; their molecular identities are, however, still subject to investigation, Recently, two type III NaPi transporters, Pit1 and Pit2, were identified, both of which exhibit some of the biochemical and regulatory characteristics of osteoblastic cell-associated NaPi transporters. Here, we have investigated the Pit1 and Pit2 steady-state mRNA Levels during the osteoblast differentiation in cultures of the nontransformed MC3T3-E1 cell line. While Pit2 mRNAs were invariably expressed at low levels, Pit1 mRNA levels were found to increaseduring osteoblast differentiation concomitantly with osteocalcin mRNA, Moreover, the increase in Pit1 mRNA levels also correlated with the time in culture at which mineralization could he observed. The increase in Pit1 mRNA levels over time in culture was only observed in cultures grown under conditions allowing for osteoblast differentiation. This is the first time that osteoblast differentiation-dependent regulation of expression of a NaPi transporter has been demonstrated. Moreover, we show here for the first time the presence of Pit1 and Pit2 mRNAs in undifferentiated and differentiated nontransformed osteoblastic cells. Our data suggest that both Pit1 and Pit2 NaPi transporters are involved in Pi transport in preosteoblastic and osteoblastic cells, and they represent the first evidence consistent with a potential role for Pit1, but not for Pit2, in differentiation-dependent Pi transport. The observed upregulation of Pit1 mRNA levels during osteoblast differentiation suggests that Pit1 might be used as a marker for osteoblast maturation. (Bone 28: 160-166; 2001) (C) 2001 by Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/02/20 alle ore 21:17:45