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Titolo:
Cellular resistance to mitomycin C is associated with overexpression of MDR-1 in a urothelial cancer cell line (MGH-U1)
Autore:
Hayes, MC; Birch, BR; Cooper, AJ; Primrose, JN;
Indirizzi:
Univ Southampton, Southampton Gen Hosp, Dept Urol, Southampton SO16 6YD, Hants, England Univ Southampton Southampton Hants England SO16 6YD 6 6YD, Hants, England Univ Southampton, Southampton Gen Hosp, Dept Surg, Southampton SO16 6YD, Hants, England Univ Southampton Southampton Hants England SO16 6YD 6 6YD, Hants, England
Titolo Testata:
BJU INTERNATIONAL
fascicolo: 3, volume: 87, anno: 2001,
pagine: 245 - 250
SICI:
1464-4096(200102)87:3<245:CRTMCI>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
MULTIDRUG-RESISTANCE; P-GLYCOPROTEIN; INTRAVESICAL CHEMOTHERAPY; CROSS-RESISTANCE; DRUG-RESISTANCE; BLADDER-CANCER; GENE; EXPRESSION; ESTABLISHMENT; TRANSPORTER;
Keywords:
multidrug resistance; MDR-1; mitomycin C; bladder cancer;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Hayes, MC Univ Southampton, Southampton Gen Hosp, Dept Urol, Tremona Rd, SouthamptonSO16 6YD, Hants, England Univ Southampton Tremona Rd Southampton Hants England SO16 6YD d
Citazione:
M.C. Hayes et al., "Cellular resistance to mitomycin C is associated with overexpression of MDR-1 in a urothelial cancer cell line (MGH-U1)", BJU INT, 87(3), 2001, pp. 245-250

Abstract

Objective To compare multidrug resistance (MDR)-1 and MDR-3 gene expression in a new urothelial cancer cell line (MGHU-1, with resistance to mitomycin C) against controls and the established (epirubicin-resistant) MDR clone,and to correlate MDR with cytotoxicity data. Materials and methods Resistance to mitomycin C was induced by the long-term exposure of wild-type MGHU-1 cells to increasing concentrations (20-400 nmol/L) of mitomycin C. The cytotoxicity of mitomycin C or epirubicin was then compared in MGHU-1, MGHU-MMC (mitomycin C-resistant) and MGHU-1R (established MDR) cells, using the tetrazolium biomass assay. The expression of MDR-1 and -3 was investigated by the reverse transcriptase-polymerase chain reaction, using cDNA-specific primers after titration, and compared with DNA and negative controls. Results MDR-1 and -3 were significantly and equally overexpressed in MGHU-1R, and associated with a dramatic increase in the 50% inhibitory drug concentration (P < 0.001) for mitomycin C and epirubicin against controls. In MGHU-MMC, the overexpression of MDR-1 was three times greater than that of MDR-3. The cytotoxicity profile for both agents was very similar to that of MGHU-1R. Trace amounts of MDR-1, but not MDR-3, were identified in the MGHU-1 wild-type. Conclusions Urothelial cancer cell resistance to mitomycin C is associatedwith cross-resistance to epirubicin and overexpression of MDR-1, suggesting that mitomycin C falls within the MDR category. Clinical application of this methodology may allow patients to be identified who are unlikely to benefit from intravesical chemotherapy.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 01:11:36