Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Evaluation of an in vitro endothelialized vascular graft under pulsatile shear stress with a novel radiolabeling procedure
Autore:
Fernandez, P; Bareille, R; Conrad, V; Midy, D; Bordenave, L;
Indirizzi:
Univ Bordeaux 2, Biophys Lab, F-33076 Bordeaux, France Univ Bordeaux 2 Bordeaux France F-33076 ys Lab, F-33076 Bordeaux, France INSERM, U443, F-33076 Bordeaux, France INSERM Bordeaux France F-33076INSERM, U443, F-33076 Bordeaux, France Hop Pellegrin, Serv Chirurg Vasc, F-33076 Bordeaux, France Hop Pellegrin Bordeaux France F-33076 urg Vasc, F-33076 Bordeaux, France
Titolo Testata:
BIOMATERIALS
fascicolo: 7, volume: 22, anno: 2001,
pagine: 649 - 658
SICI:
0142-9612(200104)22:7<649:EOAIVE>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
FIBROBLAST GROWTH-FACTOR; SMOOTH-MUSCLE CELLS; IN-VITRO; ARTERIAL PROSTHESIS; DACRON GRAFTS; HEPARIN; PROLIFERATION; SURFACE; LINE; SEQUENCE;
Keywords:
endothelial cell; vascular graft; shear stress; radioactive labeling;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Fernandez, P Univ Bordeaux 2, Biophys Lab, 146 Rue Leo Saignat, F-33076 Bordeaux, France Univ Bordeaux 2 146 Rue Leo Saignat Bordeaux France F-33076
Citazione:
P. Fernandez et al., "Evaluation of an in vitro endothelialized vascular graft under pulsatile shear stress with a novel radiolabeling procedure", BIOMATERIAL, 22(7), 2001, pp. 649-658

Abstract

Objective: To improve the hemocompatibility of vascular grafts, endothelial cell (EC) seeding of biomaterials prior to implantation is critical. The current in vitro study was designed to investigate such a feasibility on a collagen-coated heparin-bonded graft and to evaluate cell detachment upon pulsatile shear stress. Materials and methods: Endothelial cells (EA-hy-926)were seeded onto grafts. The endothelialization of the grafts was evaluated by the [H-3]-thymidine incorporation, scanning electron microscopy (SEM) and histological examinations. After in situ EC radiolabeling with a novel Tc-99m technique, the prostheses were exposed to pulsatile shear stress (0.27 N/m(2)), mimicking the shear rate occurring in a superficial femoral artery, for 3 h in a flow circuit and EC loss quantified by gamma camera detection. Results: Complete EC coverage was achieved after 5 days. Three hours of artificial perfusion resulted in a low EC loss (12.9 +/- 0.8 %, n = 7). SEM shows EC withstanding shear stress in valleys of prosthesis circumvolutions. Conclusions: These satisfactory results could be explained by the high affinity of EC For heparinized surfaces in addition to cell surface receptors involved in adhesion to collagen. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/21 alle ore 03:41:26