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Titolo:
Changes in the kinetic parameters of hepatic gamma-glutamyltransferase from streptozotocin-induced diabetic rats
Autore:
Cornwell, PD; Watkins, JB;
Indirizzi:
Indiana Univ, Sch Med, Bloomington, IN 47405 USA Indiana Univ BloomingtonIN USA 47405 Sch Med, Bloomington, IN 47405 USA
Titolo Testata:
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY
fascicolo: 1-2, volume: 1545, anno: 2001,
pagine: 184 - 191
SICI:
0167-4838(20010209)1545:1-2<184:CITKPO>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLUTAMYL-TRANSFERASE TRANSPEPTIDASE; CATALYTIC MECHANISM; LIGHT SUBUNIT; AMINO-ACIDS; GLYCOSYLATION; GLUTATHIONE; DIPEPTIDES; DATABASE; RESIDUES; ENZYME;
Keywords:
gamma-glutamyltransferase; enzyme kinetics; diabetes mellitus; streptozotocin; glycosyltransferase; glycosidase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Watkins, JB Indiana Univ, Sch Med, Jordan Hall 105, Bloomington, IN 47405 USA Indiana Univ Jordan Hall 105 Bloomington IN USA 47405 7405 USA
Citazione:
P.D. Cornwell e J.B. Watkins, "Changes in the kinetic parameters of hepatic gamma-glutamyltransferase from streptozotocin-induced diabetic rats", BBA-PROT ST, 1545(1-2), 2001, pp. 184-191

Abstract

Previous research has shown that the enzymatic activity of hepatic gamma -glutamyltransferase was increased in streptozotocin-induced diabetic rats with no increase in the expression of the protein. The current work has characterized the differences in the kinetic properties of hepatic gamma -glutamyltransferase from diabetic versus control rats. Hepatic gamma -glutamyltransferase was purified from control male and female rats and from rats madediabetic 30 days previously with streptozotocin. The maximal velocity and the Michaelis constant were determined for the purified enzyme with two separate donors (L-gamma -glutamyl-p-nitroanilide or L-gamma -glutamyl-(7-amido-4-methylcoumarin)) in the presence of one of eight accepters ((L)-alanine-glycine, L-glycine-glycine, L-methionine, L-glutamate, L-alanine, L-glutamine, L-phenylalanine or (L)-aspartate). With both donors, hepatic gamma -glutamyltransferase from diabetic rats had a consistently higher kinetic efficiency than gamma -glutamyltransferase from controls. The kinetic efficiency percent increase of diabetic over control gamma -glutamyltransferase whenaveraged across all accepters was higher in males than in females. With (L)-gamma -glutamyl-p-nitroanilide, the kinetic efficiency increase of diabetic over control gamma -glutamyltransferase was higher with poor accepters than with highly efficient accepters. These data indicate that there are differences in the physical properties of hepatic gamma -glutamyltransferase from diabetic versus control rats and from female versus male rats. (C) 2001Elsevier Science B.V. All rights reserved.

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Documento generato il 14/07/20 alle ore 05:40:05