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Titolo:
The phorbol ester PMA and cyclic AMP activate different Cl- and HCO3- fluxes in C127 cells expressing CFTR
Autore:
Zegarra-Moran, O; Porcelli, AM; Rugolo, M;
Indirizzi:
Univ Bologna, Dipartimento Biol Ev Sp, I-40126 Bologna, Italy Univ Bologna Bologna Italy I-40126 to Biol Ev Sp, I-40126 Bologna, Italy Ist Giannina Gaslini, Genet Mol Lab, I-16148 Genoa, Italy Ist Giannina Gaslini Genoa Italy I-16148 t Mol Lab, I-16148 Genoa, Italy
Titolo Testata:
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
fascicolo: 2, volume: 1535, anno: 2001,
pagine: 120 - 127
SICI:
0925-4439(20010214)1535:2<120:TPEPAC>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSMEMBRANE CONDUCTANCE REGULATOR; CYSTIC-FIBROSIS GENE; CHLORIDE CURRENTS; CHANNEL; PHOSPHORYLATION; MECHANISMS;
Keywords:
cystic fibrosis; CFTR; HCO3- influx; phorbol ester (PMA); swelling-activated chloride current; patch clamp;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Rugolo, M Univ Bologna, Dipartimento Biol Ev Sp, Via Irnerio 42, I-40126 Bologna, Italy Univ Bologna Via Irnerio 42 Bologna Italy I-40126 ologna, Italy
Citazione:
O. Zegarra-Moran et al., "The phorbol ester PMA and cyclic AMP activate different Cl- and HCO3- fluxes in C127 cells expressing CFTR", BBA-MOL BAS, 1535(2), 2001, pp. 120-127

Abstract

In mouse mammary epithelial C127 cells expressing wild-type cystic fibrosis transmembrane conductance regulator (CFTR), chloride efflux, measured with the Cl--sensitive dye 6-methoxy-N-(3-sulfopropyl)quinolinium (SPQ), was stimulated by activation of protein kinase A with cyclic AMP elevating agents forskolin plus 3-isobutyl-1-methyl-xanthine (IBMX) and, to a less extent,by activation of protein kinase C with the phorbol 12-myristate 13-acetate(PMA). Conversely, bicarbonate influx, determined by intracellular alkalinization of cells incubated with the pH-sensitive dye 2',7'-bis-(2-carboxyethyl)-5(6)carboxyfluoresceintetraacetoxymethyl ester (BCECF-AM), was stimulated by cyclic AMP elevation, but not by PMA. Patch clamp analysis revealed that PMA activated a Cl- current with the typical biophysical characteristics of swelling-activated current and not of CFTR. (C) 2001 Elsevier ScienceB.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 21:04:33