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Titolo:
Comparative study of blood group-recognizing lectins toward ABO blood group antigens on neoglycoproteins, glycoproteins and complex-type oligosaccharides
Autore:
Matsui, T; Hamako, J; Ozeki, Y; Titani, K;
Indirizzi:
Fujita Hlth Univ, Div Biomed Polymer Sci, Inst Comprehens Med Sci, Toyoake, Aichi 4701192, Japan Fujita Hlth Univ Toyoake Aichi Japan 4701192 oyoake, Aichi 4701192, Japan Fujita Hlth Univ Coll, Dept Med Informat Technol, Toyoake, Aichi 4701192, Japan Fujita Hlth Univ Coll Toyoake Aichi Japan 4701192 e, Aichi 4701192, Japan Yokohama City Univ, Fac Sci, Dept Elemental Sci, Yokohama, Kanagawa 2360027, Japan Yokohama City Univ Yokohama Kanagawa Japan 2360027 anagawa 2360027, Japan
Titolo Testata:
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
fascicolo: 1-2, volume: 1525, anno: 2001,
pagine: 50 - 57
SICI:
0304-4165(20010216)1525:1-2<50:CSOBGL>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN VONWILLEBRAND-FACTOR; VON-WILLEBRAND-FACTOR; ASPARAGINE-LINKED OLIGOSACCHARIDES; AFFINITY-CHROMATOGRAPHY; HUMAN PLASMA; GROUP-A; CIRCULATING GLYCOPROTEINS; GROUP-B; GROUP-H; BINDING;
Keywords:
ABO blood group; lectin; complex-type oligosaccharide; von Willebrand factor; Helix pomatia agglutinin; Ulex europaeus agglutinin I;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Matsui, T Fujita Hlth Univ, Div Biomed Polymer Sci, Inst Comprehens Med Sci, Toyoake, Aichi 4701192, Japan Fujita Hlth Univ Toyoake Aichi Japan 4701192 chi 4701192, Japan
Citazione:
T. Matsui et al., "Comparative study of blood group-recognizing lectins toward ABO blood group antigens on neoglycoproteins, glycoproteins and complex-type oligosaccharides", BBA-GEN SUB, 1525(1-2), 2001, pp. 50-57

Abstract

Binding specificities of ABO blood group-recognizing lectins toward blood group antigens on neoglycoproteins, glycoproteins and complex-type oligosaccharides were studied by lectin-blotting analysis, enzyme linked immunosorbent assay and lectin-conjugated agarose column chromatography. Human serum albumin conjugated with A- and B-trisaccharides was clearly recognized by Helix pomatia (WA), Phaseolus lunatus, Dolichos biflorus agglutinins, and Griffonia simplicifolia I agglutinin B-4, respectively. Almost the same results were obtained for human group A and B ovarian cyst and A-active hog gastric mucins, but Glycine max agglutinin only reacted to the group A hog mucin. When human plasma von Willebrand factor (VWF), having Asn-linked blood group antigens, was tested, HPA was highly sensitive to blood group A antigen on the VWF. Ulex europaeus agglutinin I (UEA-I) preferentially bound to the vWF from blood group O plasma. Within the GalNAc-recognizing lectins examined, a biantennary complex-type oligosaccharide having the blood group A structure retarded on an HPA-agarose column, and the affinity was diminished after digestion with alpha -N-acetylgalactosaminidase. This product boundto UEA-I agarose column. These results indicate that HPA and UEA-I are most sensitive for detection of glycoproteins possessing small amounts of blood group A and H antigens and also useful for fractionation of complex-type oligosaccharides with blood group A and H antigens, respectively. (C) 2001 Elsevier Science B.V. All rights reserved.

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Documento generato il 23/09/20 alle ore 12:52:31