Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Analysis of anti-rotavirus activity of extract from Stevia rebaudiana
Autore:
Takahashi, K; Matsuda, M; Ohashi, K; Taniguchi, K; Nakagomi, O; Abe, Y; Mori, S; Sato, N; Okutani, K; Shigeta, S;
Indirizzi:
Fukushima Med Univ, Sch Med, Dept Microbiol, Fukushima 9601295, Japan Fukushima Med Univ Fukushima Japan 9601295 iol, Fukushima 9601295, Japan Kagawa Univ, Fac Agr, Dept Biochem & Food Sci, Kagawa, Japan Kagawa Univ Kagawa Japan ac Agr, Dept Biochem & Food Sci, Kagawa, Japan Fujita Hlth Univ, Sch Med, Dept Virol & Parasitol, Aichi, Japan Fujita Hlth Univ Aichi Japan Med, Dept Virol & Parasitol, Aichi, Japan Akita Univ, Sch Med, Dept Microbiol, Akita 010, Japan Akita Univ Akita Japan 010 iv, Sch Med, Dept Microbiol, Akita 010, Japan
Titolo Testata:
ANTIVIRAL RESEARCH
fascicolo: 1, volume: 49, anno: 2001,
pagine: 15 - 24
SICI:
0166-3542(200101)49:1<15:AOAAOE>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
3-DIMENSIONAL STRUCTURE; NEUTRALIZATION EPITOPES; CRYOELECTRON MICROSCOPY; MONOCLONAL-ANTIBODIES; BOVINE ROTAVIRUS; CELL ATTACHMENT; SUCKLING MICE; IN-VITRO; VIRUS; SEQUENCE;
Keywords:
Stevia rebaudiana; polysaccharide; anti-rotavirus activity; virus binding; VP7;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Takahashi, K Fukushima Med Univ, Sch Med, Dept Microbiol, 1 Hikarigaoka, Fukushima 9601295, Japan Fukushima Med Univ 1 Hikarigaoka Fukushima Japan 9601295 pan
Citazione:
K. Takahashi et al., "Analysis of anti-rotavirus activity of extract from Stevia rebaudiana", ANTIVIR RES, 49(1), 2001, pp. 15-24

Abstract

Anti-human rotavirus (HRV) activity of hot water extracts from Stevia rebaudiana (SE) was examined. SE inhibited the replication of all four serotypes of HRV in vitro. This inhibitory effect of SE was not reduced on the prior exposure of SE to HCl for 30 min at pH 2. Binding assay with radiolabeledpurified viruses indicated that the inhibitory mechanism of SE is the blockade of virus binding. The SE inhibited the binding of anti-VP7 monoclonal antibody to HRV-infected MA104 cells. The inhibitory components of SE were found to be heterogeneous anionic polysaccharides with different ion charges. The component analyses suggested that the purified Fraction named as Stevian with the highest inhibitory activity consists of the anionic polysaccharide with molecular weight of 9800, and contains Ser and Ala as amino acids. Analyses of sugar residues suggest uronic acid(s) as sugar components. it did not contain amino and neutral sugars and sulfate residues. These findings suggest that SE may bind to 37 kD VP7 and interfere with the binding of VP7 to the cellular receptors by steric: hindrance, which results in the blockade of the virus attachment to cells. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 02:41:56