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Titolo:
Multispectral imaging microscope with millisecond time resolution
Autore:
Khait, O; Smirnov, S; Tran, CD;
Indirizzi:
Marquette Univ, Dept Chem, Milwaukee, WI 53201 USA Marquette Univ Milwaukee WI USA 53201 Dept Chem, Milwaukee, WI 53201 USA
Titolo Testata:
ANALYTICAL CHEMISTRY
fascicolo: 4, volume: 73, anno: 2001,
pagine: 732 - 739
SICI:
0003-2700(20010215)73:4<732:MIMWMT>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
FLOW-INJECTION ANALYSIS; TUNABLE FILTERS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
17
Recensione:
Indirizzi per estratti:
Indirizzo: Tran, CD Marquette Univ, Dept Chem, POB 1881, Milwaukee, WI 53201 USA Marquette Univ POB 1881 Milwaukee WI USA 53201 ukee, WI 53201 USA
Citazione:
O. Khait et al., "Multispectral imaging microscope with millisecond time resolution", ANALYT CHEM, 73(4), 2001, pp. 732-739

Abstract

A new multispectral imaging microscope with micrometer spatial resolution and millisecond temporal resolution has been developed. The imaging microscope is based on the use of an acousto-optic tunable filter (AOTF) for spectral tuning and a progressive scan camera capable of snapshot operation for recording, It can operate in two modes: images are recorded as a function of time or wavelength. When operated as a function of time, the microscope is configured so that as many images as possible are recorded, grabbed, and stored per one wavelength. Upon completion, the AOTF is scanned to a new wavelength, and a new set of images are recorded, Up to 33 images/second (i.e., 30 ms/image) can be recorded in this mode. In the other configuration, the recording wavelength is rapidly scanned (by means of the AOTF) and only one image is rapidly recorded, grabbed, and stored for each wavelength. Because additional time is needed to scan the AOTF, the maximum number of images can be grabbed in this case is 16 frames/s, Preliminary applications of the imaging microscope include measurements of photoinduced changes of a single unit cell in temperature-sensitive cholesteric liquid crystals as a function of time and wavelength. The changes were found to be varied with time and wavelength. Interestingly, the photoinduced changes of unit cells in the liquid crystal are not the same but different from cell to cell. This imaging microscope is particularly useful for measurements of small-size samples that undergo rapid chemical or biochemical reactions, e.g,, activitiesof a single biological cell.

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Documento generato il 28/11/20 alle ore 11:33:46