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Titolo:
Microvessel density counting in breast cancer - Slides vs. digital images
Autore:
Cruz, D; Valenti, C; Dias, A; Seixas, M; Schmitt, F;
Indirizzi:
Univ Porto, Inst Patol & Imunol Mol, P-4200 Oporto, Portugal Univ Porto Oporto Portugal P-4200 & Imunol Mol, P-4200 Oporto, Portugal
Titolo Testata:
ANALYTICAL AND QUANTITATIVE CYTOLOGY AND HISTOLOGY
fascicolo: 1, volume: 23, anno: 2001,
pagine: 15 - 20
SICI:
0884-6812(200102)23:1<15:MDCIBC>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR ANGIOGENESIS; VESSEL INVASION; CARCINOMA; SIZE;
Keywords:
breast neoplasms; blood vessels; capillaries; microvessel density counting;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Cruz, D Univ Porto, Inst Patol & Imunol Mol, Rua Dr Roberto Frias S-N, P-4200 Oporto, Portugal Univ Porto Rua Dr Roberto Frias S-N Oporto Portugal P-4200 rtugal
Citazione:
D. Cruz et al., "Microvessel density counting in breast cancer - Slides vs. digital images", ANAL QUAN C, 23(1), 2001, pp. 15-20

Abstract

OBJECTIVE: To develop a program to assist the pathologist in the acquisition and evaluation of digital images to determine microvessel density (MVD) in tissues. STUDY DESIGN: Ten cases of breast cancer with a high degree of neovascularization were selected. A standard immunohistochemical method teas used to highlight the microvessels (monoclonal anti-factor VIII, avidin-biotin-peroxidase complex method). Two pathologists (one senior [S] and one junior [J])evaluated four areas of highest neovascularization ("hot spots") in the tumors. Microscopically MVD was determined in four chosen areas (400:2). Fromthe center of each area two digital images were acquired at a magnification of 200:1. All counts made by microscopic observation were compared with those made on the digital images. To compare MVD counting at different resolution, two sets of images at different sampling densities (320 x 240 and 1,600 x 2,200) were assessed by the two pathologists. RESULTS: We obtained a good correlation (r = .98 for S and .96 for J) between the MVD counts obtained at the microscope (192.8 MV/mm(2) [mean of S] and 181.8 MV/mm(2) [mean of J]) and the MVD counts from digital images (153.2 MV/mm(2) [mean of S] and 171.0 MV/mm(2) [mean of J]) at high resolution. The counts were lower for digital images at lower sampling density (125.0 MV/mm(2) [mean of S] and 78.2 MV/mm(2) [mean of J]). With low-resolution digital images only S maintained a good correlation (r = .96 for S and .34 forJ) with the microscopic evaluation of MVD. Interobserver analysis showed agood correlation (r = .82 for the microscope and r = .78 for the digital images) of MVD evaluated either at the microscope or in high-resolution digital images. CONCLUSION: We demonstrated the functionality and usefulness of our program in performing MVD evaluation. Considering the capabilities of the programto store all images and microvessel marks and the reliability of MVD evaluation based on digital images, we consider this program the first step toward fully automated MVD assessment.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 13:20:20