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Titolo:
Topical application of antiangiogenic agent AGM-1470 suppresses anastomotic intimal hyperplasia after ePTFE grafting in a rabbit model
Autore:
Shigematsu, K; Yasuhara, H; Shigematsu, H;
Indirizzi:
Univ Tokyo, Dept Surg, Div Vasc Surg, Bunkyo Ku, Tokyo 1138655, Japan UnivTokyo Tokyo Japan 1138655 asc Surg, Bunkyo Ku, Tokyo 1138655, Japan Teikyo Univ, Ichihara Hosp, Dept Surg, Tokyo 173, Japan Teikyo Univ Tokyo Japan 173 , Ichihara Hosp, Dept Surg, Tokyo 173, Japan
Titolo Testata:
SURGERY
fascicolo: 2, volume: 129, anno: 2001,
pagine: 220 - 230
SICI:
0039-6060(200102)129:2<220:TAOAAA>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANGIOGENESIS INHIBITOR TNP-470; PERFORMANCE LIQUID-CHROMATOGRAPHY; MUSCLE CELL-PROLIFERATION; GROWTH-FACTOR PRODUCTION; TUMOR-GROWTH; VEIN GRAFTS; POLYTETRAFLUOROETHYLENE GRAFTS; POSSIBLE INVOLVEMENT; DACRON GRAFTS; CANINE MODEL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Shigematsu, H Univ Tokyo, Dept Surg, Div Vasc Surg, Bunkyo Ku, 7-3-1 Hongo, Tokyo 1138655, Japan Univ Tokyo 7-3-1 Hongo Tokyo Japan 1138655 o 1138655, Japan
Citazione:
K. Shigematsu et al., "Topical application of antiangiogenic agent AGM-1470 suppresses anastomotic intimal hyperplasia after ePTFE grafting in a rabbit model", SURGERY, 129(2), 2001, pp. 220-230

Abstract

Background. Anastomotic intimal hyperplasia (AIH) remains an unsolved problem. Angiogenesis around the anastomosis one of the important mechanisms accelerating AIH. In this study, we investigated the effects of an antiangiogenic agent AGM-1470 (O-[chloroacetyl-carbamoyl] fumagillol: AGM) on the thickness of AIH after expanded polytetrafluoroethylene grafting. Methods. Study 1: Smooth muscle cells (SMCs) were cultured to form 3-mm-side square colonies by using 4 kinds of culture medium, containing AGM at concentrations of 0, 0.1, 1.0, and 10 ng/mL. The SMC colony spreading distance in each group was measured as an index of mitogenic activity. The isolated proliferative activity of SMCs was also assessed. Study 2: Male New Zealand white rabbits underwent inlay expanded polytetrafluoroethylene grafting of the carotid arteries. They were divided in 4 groups (control, vehicle, AGM [0.5], and AGM [5]) in which no topical application, Vaseline ointment, Vaseline ointment containing 0.5 mg AGM, or Vaseline ointment containing 5 mg AGM was applied to the anastomoses, respectively. Rabbits were fed a high-cholesterol diet for 2 weeks before and 8 weeks after the operation. AIH thickness was measured and capillary formation and SMC accumulation around the anastomoses were examined with immunohistochemical staining. Results. Study 1: AGM suppressed SMC migratory activity in cytostatic, butnot cytotoxic, manner. Study 2: AGM ointment inhibited AIH in proportion to it concentration and also suppressed new capillary formation around the anastomoses and SMC accumulation in AIH. Conclusions. Topical application of the antiangiogenic agent AGM may become an important strategy for preventing AIH.

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Documento generato il 05/04/20 alle ore 03:53:04