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Titolo:
The 100-kDa U5 snRNP protein (hPrp28p) contacts the 5 ' splice site through its ATPase site
Autore:
Ismaili, N; Sha, M; Gustafson, EH; Konarska, MM;
Indirizzi:
Rockefeller Univ, New York, NY 10021 USA Rockefeller Univ New York NY USA10021 eller Univ, New York, NY 10021 USA
Titolo Testata:
RNA-A PUBLICATION OF THE RNA SOCIETY
fascicolo: 2, volume: 7, anno: 2001,
pagine: 182 - 193
SICI:
1355-8382(200102)7:2<182:T1USP(>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
PRE-MESSENGER-RNA; DEAD-BOX PROTEIN; DOMINANT-NEGATIVE MUTATION; IN-VITRO; SR PROTEINS; SACCHAROMYCES-CEREVISIAE; GEL-ELECTROPHORESIS; CRYSTAL-STRUCTURES; DEPENDENT ATPASE; HELICASE DOMAIN;
Keywords:
5 ' splice site; pre-mRNA splicing; Prp28; RNA helicase; spliceosome;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
54
Recensione:
Indirizzi per estratti:
Indirizzo: Konarska, MM Rockefeller Univ, 1230 York Ave, New York, NY 10021 USA Rockefeller Univ 1230 York Ave New York NY USA 10021 0021 USA
Citazione:
N. Ismaili et al., "The 100-kDa U5 snRNP protein (hPrp28p) contacts the 5 ' splice site through its ATPase site", RNA, 7(2), 2001, pp. 182-193

Abstract

To identify splicing factors in proximity of the 5' splice site (5'SS), wefollowed a crosslinking profile of site-specifically modified, photoreactive RNA substrates. Upon U4/U5/U6 snRNP addition, the 5'SS RNA crosslinks inan ATP-dependent manner to U6 snRNA, an unidentified protein p27, and the 100-kDa U5 snRNP protein, a human ortholog of an ATPase/RNA helicase yPrp28p. The 5'SS:hPrp28p crosslink maps to the highly conserved TAT motif in proximity of the ATP-binding site in hPrp28p. We propose that hPrp28p acts as a helicase to unwind the 5'SS:U1 snRNA duplex, and at the same time as a 5'SS translocase, which, upon NTP-dependent conformational change, positions the 5'SS for pairing with U6 snRNA within the spliceosome. This repositioning of the 5'SS takes place regardless of whether the 5'SS is originally duplexed with U1 snRNA.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 08:21:52