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Titolo:
Rapid evaluation and optimization of recombinant protein production using GFP tagging
Autore:
Rucker, E; Schneider, G; Steinhauser, K; Lower, R; Hauber, J; Stauber, RH;
Indirizzi:
Univ Erlangen Nurnberg, Inst Med & Clin Virol, D-91054 Erlangen, Germany Univ Erlangen Nurnberg Erlangen Germany D-91054 -91054 Erlangen, Germany Paul Ehrlich Inst, D-63225 Langen, Germany Paul Ehrlich Inst Langen Germany D-63225 h Inst, D-63225 Langen, Germany
Titolo Testata:
PROTEIN EXPRESSION AND PURIFICATION
fascicolo: 1, volume: 21, anno: 2001,
pagine: 220 - 223
SICI:
1046-5928(200102)21:1<220:REAOOR>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
GREEN FLUORESCENT PROTEIN; LIVING CELLS; MUTANTS;
Keywords:
recombinant protein production; GFP; GST;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
7
Recensione:
Indirizzi per estratti:
Indirizzo: Stauber, RH Univ Erlangen Nurnberg, Inst Med & Clin Virol, Schlossgarten 4, D-91054 Erlangen, Germany Univ Erlangen Nurnberg Schlossgarten 4 Erlangen Germany D-91054
Citazione:
E. Rucker et al., "Rapid evaluation and optimization of recombinant protein production using GFP tagging", PROT EX PUR, 21(1), 2001, pp. 220-223

Abstract

The isolation of recombinant proteins from bacterial or eukaryotic systemsoften requires a laborious optimization of expression and purification conditions. To greatly facilitate this procedure we included the green fluorescent protein (GFP) in bacterial expression vectors. This approach allowed us to sensitively detect the GFP hybrid proteins already in intact bacterialcells using a fluorescence microscope. To rapidly analyze a variety of conditions essential for protein expression, the GFP signal, indicative of expression levels, was directly quantitated in live bacterial suspensions using a fluorescence plate reader. Thus, GFP tagging not only allows one to directly monitor protein expression in general but also appears to increase protein stability or solubility. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 21:55:12