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Titolo:
A second-generation vaccine protects against the psychoactive effects of cocaine
Autore:
Carrera, MRA; Ashley, JA; Wirsching, P; Koob, GF; Janda, KD;
Indirizzi:
Scripps Clin & Res Fdn, Res Inst, Dept Chem & Mol Biol, La Jolla, CA 92037USA Scripps Clin & Res Fdn La Jolla CA USA 92037 Biol, La Jolla, CA 92037USA Scripps Clin & Res Fdn, Res Inst, Dept Neuropharmacol, La Jolla, CA 92037 USA Scripps Clin & Res Fdn La Jolla CA USA 92037 acol, La Jolla, CA 92037 USA Skaggs Inst chem Biol, La Jolla, CA 92037 USA Skaggs Inst chem Biol La Jolla CA USA 92037 Biol, La Jolla, CA 92037 USA
Titolo Testata:
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
fascicolo: 4, volume: 98, anno: 2001,
pagine: 1988 - 1992
SICI:
0027-8424(20010213)98:4<1988:ASVPAT>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACTIVE IMMUNIZATION; ABUSE; PHARMACOKINETICS; DEPENDENCE; ANTIGEN; FUSION; LINES; RATS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Janda, KD Scripps Clin & Res Fdn, Res Inst, Dept Chem & Mol Biol, 10550 N Torrey Pines Rd, La Jolla, CA 92037 USA Scripps Clin & Res Fdn 10550 N Torrey Pines Rd La Jolla CA USA 92037
Citazione:
M.R.A. Carrera et al., "A second-generation vaccine protects against the psychoactive effects of cocaine", P NAS US, 98(4), 2001, pp. 1988-1992

Abstract

The effects of immunization with the second-generation cocaine immunoconjugate GND-keyhole limpet hemocyanin (KLH) or with the anti-cocaine mAb GNC92H2 were assessed in a model of acute cocaine-induced locomotor activity. After i.p. administration of cocaine . HCI (15 mg/kg), rats were tested in photocell cages, and stereotypy was rated to determine preimmunization drug response (baseline). Experimental animals were subjected to an immunization protocol with GND-KLH or treated with the mAb GNC92H2. Rats were then challenged with systemic cocaine, and their locomotor responses were again measured. Active immunization with GND-KLH produced a 76% decrease in the ambulatory measure (crossovers) in the experimental group and a 12% increase in the control group compared with baseline values. Also, stereotypic behavior was significantly suppressed in the vaccinated animals. Decreases in both measures were seen in the experimental group on two subsequent challenges. The maximum effect was observed at the time of the second challenge with a dramatic 80% decrease in crossovers. Treatment with GNC92H2 resulted in a 69% decrease in crossovers compared with baseline. This effect persisted across two additional challenges over 11 days with decreases of 46-47%. In contrast, the control group showed increases of up to 28%. Significant differences between groups were observed in the stereotypic measure in all three challenges. The results indicate that these immunopharmacotherapeutic agents have significant cocaine-blockade potential and therefore may offer an effective strategy for the treatment of cocaine abuse.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 16:37:03