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Titolo:
Plastid-expressed 5-enolpyruvylshikimate-3-phosphate synthase genes provide high level glyphosate tolerance in tobacco
Autore:
Ye, GN; Hajdukiewicz, PTJ; Broyles, D; Rodriguez, D; Xu, CW; Nehra, N; Staub, JM;
Indirizzi:
Monsanto Co, St Louis, MO 63198 USA Monsanto Co St Louis MO USA 63198Monsanto Co, St Louis, MO 63198 USA
Titolo Testata:
PLANT JOURNAL
fascicolo: 3, volume: 25, anno: 2001,
pagine: 261 - 270
SICI:
0960-7412(200102)25:3<261:P5SGP>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESCHERICHIA-COLI; HERBICIDE GLYPHOSATE; MESSENGER-RNA; EPSP SYNTHASE; FOREIGN GENES; CHLOROPLASTS; PROTEIN; PLANTS; TRANSFORMATION; GENOME;
Keywords:
chloroplast; herbicide; maternal inheritance; plastid; transformation; transplastomic;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Staub, JM Monsanto Co, 700 Chesterfield Village Pkwy N, St Louis, MO 63198USA Monsanto Co 700 Chesterfield Village Pkwy N St Louis MO USA 63198
Citazione:
G.N. Ye et al., "Plastid-expressed 5-enolpyruvylshikimate-3-phosphate synthase genes provide high level glyphosate tolerance in tobacco", PLANT J, 25(3), 2001, pp. 261-270

Abstract

Plastid transformation (transplastomic) technology has several potential advantages for biotechnological applications including the use of unmodifiedprokaryotic genes for engineering, potential high-level gene expression and gene containment due to maternal inheritance in most crop plants. However, the efficacy of a plastid-encoded trait may change depending on plastid number and tissue type. We report a feasibility study in tobacco plastids toachieve high-level herbicide resistance in both vegetative tissues and reproductive organs. We chose to test glyphosate resistance via over-expression in plastids of tolerant forms of 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS). Immunological, enzymatic and whole-plant assays were used to prove the efficacy of three different prokaryotic (Achromobacter, Agrobacterium and Bacillus) EPSPS genes. Using the Agrobacterium strain CP4 EPSPS as a model we identified translational control sequences that direct a 10 000-fold range of protein accumulation (to > 10% total soluble protein in leaves). Plastid-expressed EPSPS could provide very high levels of glyphosate resistance, although levels of resistance in vegetative and reproductive tissues differed depending on EPSPS accumulation levels, and correlated to the plastid abundance in these tissues. Paradoxically, higher levels of plastid-expressed EPSPS protein accumulation were apparently required for efficacythan from a similar nuclear-encoded gene. Nevertheless, the demonstration of high-level glyphosate tolerance in vegetative and reproductive organs using transplastomic technology provides a necessary step for transfer of this technology to other crop species.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 10:02:40