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Titolo:
Primary chondrocytes resist hydrostatic pressure-induced stress while primary synovial cells and fibroblasts show modified Hsp70 response
Autore:
Kaarniranta, K; Holmberg, CI; Lammi, MJ; Eriksson, JE; Sistonen, L; Helminen, HJ;
Indirizzi:
Univ Kuopio, Dept Anat, Kuopio 70211, Finland Univ Kuopio Kuopio Finland70211 uopio, Dept Anat, Kuopio 70211, Finland Univ Turku, Abo Akad Univ, Turku Ctr Biotechnol, Turku 20521, Finland UnivTurku Turku Finland 20521 urku Ctr Biotechnol, Turku 20521, Finland Abo Akad Univ, Dept Biochem & Pharm, Turku 20521, Finland Abo Akad Univ Turku Finland 20521 Biochem & Pharm, Turku 20521, Finland Abo Akad Univ, Dept Biol, Turku 20521, Finland Abo Akad Univ Turku Finland 20521 Univ, Dept Biol, Turku 20521, Finland
Titolo Testata:
OSTEOARTHRITIS AND CARTILAGE
fascicolo: 1, volume: 9, anno: 2001,
pagine: 7 - 13
SICI:
1063-4584(200101)9:1<7:PCRHPS>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEAT-SHOCK; MOLECULAR CHAPERONES; ARTICULAR-CARTILAGE; GENE-TRANSCRIPTION; ANTIINFLAMMATORY DRUGS; CULTURED CHONDROCYTES; MATRIX SYNTHESIS; BINDING ACTIVITY; KNEE JOINTS; BEAGLE DOGS;
Keywords:
chondrocyte; gene regulation; heat shock protein; hydrostatic pressure;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
64
Recensione:
Indirizzi per estratti:
Indirizzo: Helminen, HJ Univ Kuopio, Dept Anat, POB 1627, Kuopio 70211, Finland Univ Kuopio POB 1627 Kuopio Finland 70211 pio 70211, Finland
Citazione:
K. Kaarniranta et al., "Primary chondrocytes resist hydrostatic pressure-induced stress while primary synovial cells and fibroblasts show modified Hsp70 response", OSTEO CART, 9(1), 2001, pp. 7-13

Abstract

Objective: During joint loading, chondrocytes in the articular cartilage are subjected to gradients of high compressive hydrostatic pressure (HP). Inresponse to diverse chemical or physical stresses, heat shock genes are induced to express heat shock proteins (Hsps). This study sought to examine the role of Hsps in baroresistance in primary bovine chondrocytes and synovial cells, as well as in primary human fibroblasts. Methods: Northern blotting was used to analyze the steady-state levels of hsp70 mRNA in the primary cells exposed to HP or heat stress. Hsp70 proteinaccumulation was analyzed by Western blotting, and the DNA-binding activity was examined by gel mobility shift assay. Results: Primary bovine chondrocytes which have been adapted to live underpressurized conditions showed negligible Hsp70 response upon HP loading, whereas primary bovine synovial cells and human fibroblasts accumulated hsp70 mRNA and protein when subjected to HP. The response was initiated withoutactivation of the heat shock transcription factor 1. Interestingly, pre-conditioning of the barosensitive fibroblasts with HP or heat shock reduced the Hsp70 response, indicating induction of baroresistance. Conclusion: This study suggests that Hsp70 can play an important role in the early stages of adaptation of cells to HP. Thus, the Hsp70 gene expression upon HP loading may serve as one indicator of the chondrocytic phenotypeof the cells. This can be of use in the treatment of cartilage lesions. (C) 2001 OsteoArthritis Research Society International.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 00:28:40