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Titolo:
Effect of a mutant form of I kappa B-alpha on 5-fluorouracil-induced apoptosis in transformed human salivary gland cells
Autore:
Motegi, K; Azuma, M; Aota, K; Yamashita, T; Tamatani, T; Harada, K; Yoshida, H; Sato, M;
Indirizzi:
Univ Tokushima, Sch Dent, Dept Oral & Maxillofacial Surg 2, Tokushima 7708504, Japan Univ Tokushima Tokushima Japan 7708504 Surg 2, Tokushima 7708504, Japan
Titolo Testata:
ORAL ONCOLOGY
fascicolo: 2, volume: 37, anno: 2001,
pagine: 185 - 192
SICI:
1368-8375(200102)37:2<185:EOAMFO>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACTIVATION; INHIBITION; CARCINOMA; PHOSPHORYLATION; EXPRESSION; INDUCTION; MECHANISM; PROTEINS; DEATH;
Keywords:
5-FU; NF-kappa B; I kappa B-alpha; human salivary gland cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Azuma, M Univ Tokushima, Sch Dent, Dept Oral & Maxillofacial Surg 2, 3 Kuramoto Cho, Tokushima 7708504, Japan Univ Tokushima 3 Kuramoto Cho Tokushima Japan 7708504 504, Japan
Citazione:
K. Motegi et al., "Effect of a mutant form of I kappa B-alpha on 5-fluorouracil-induced apoptosis in transformed human salivary gland cells", ORAL ONCOL, 37(2), 2001, pp. 185-192

Abstract

Increasing evidence indicates that transcription factor NF-kappaB may playa role in cell survival, and that some chemotherapeutic agents activate NF-kappaB, while inhibition of NF-kappaB renders cells sensitive to these drugs. 5-Fluorouracil (5-FU) exerts its cytotoxic effect through the inductionof apoptosis. However, it still remains uncertain whether 5-FU treatment in combination with the inhibition of NF-kappaB largely exerts an anti-proliferative effect on the growth of neoplastic human salivary gland cells. Thus, we investigated whether NF-kappaB suppression in transformed human salivary gland (NS-SV-AC) cells leads to a marked reduction in cell growth in response to 5-FU treatment. Our results demonstrated that under unstimulated conditions, the ability of cell growth in the super-repressor form of I kappaB-alpha (srI kappaB-alpha) cDNA-transfected cell clones (ACMT-6 and -7) was significantly lower than that in the empty vector-transfected cell clone(ACpRc-1). In addition, the growth inhibition caused by 5-FU was greatly enhanced in ACMT-6 and -7 as compared to ACpRc-1. Based on fractional inhibition analysis, this growth inhibition was due to an additive effect of bothinhibitors. Electrophoretic mobility shift assay revealed that NF-kappaB activity in these cell clones was not affected by treatment with 5-FU. Accordingly, our data provide evidence that the combination of 5-FU and NF-KB suppression cooperatively functions in the growth inhibition of NS-SV-AC cells. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 19:57:03