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Titolo:
Alternative splicing of a Drosophila GABA receptor subunit gene identifiesdeterminants of agonist potency
Autore:
Hosie, AM; Buckingham, SD; Presnail, JK; Sattelle, DB;
Indirizzi:
Univ Oxford, Dept Human Anat & Genet, MRC, Funct Genet Unit, Oxford OX1 3QX, England Univ Oxford Oxford England OX1 3QX t Genet Unit, Oxford OX1 3QX, England Univ Cambridge, Dept Zool, Babraham Inst, Mol Signalling Lab, Cambridge CB2 3EJ, England Univ Cambridge Cambridge England CB2 3EJ Lab, Cambridge CB2 3EJ, England Dupont Co, Stine Haskell Res Ctr, Newark, DE 19714 USA Dupont Co Newark DE USA 19714 Stine Haskell Res Ctr, Newark, DE 19714 USA
Titolo Testata:
NEUROSCIENCE
fascicolo: 3, volume: 102, anno: 2001,
pagine: 709 - 714
SICI:
0306-4522(2001)102:3<709:ASOADG>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
GAMMA-AMINOBUTYRIC-ACID; INSECTICIDE RESISTANCE; FUNCTIONAL EXPRESSION; CYCLODIENE RESISTANCE; NICOTINIC RECEPTORS; XENOPUS OOCYTES; ALPHA-SUBUNITS; DELTA-SUBUNIT; ION CHANNELS; ACETYLCHOLINE;
Keywords:
Rdl gene; ligand-gated ion channel D. melanogaster; gene splicing; agonist binding site;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Sattelle, DB Univ Oxford, Dept Human Anat & Genet, MRC, Funct Genet Unit, S Parks Rd, Oxford OX1 3QX, England Univ Oxford S Parks Rd Oxford England OX1 3QX 1 3QX, England
Citazione:
A.M. Hosie et al., "Alternative splicing of a Drosophila GABA receptor subunit gene identifiesdeterminants of agonist potency", NEUROSCIENC, 102(3), 2001, pp. 709-714

Abstract

Alternative splicing of the Drosophila melanogaster Rdl gene yields four ionotropic GABA receptor subunits. The two Rdl splice variants cloned to date, RDLac and RDLbd (DRC17-1-2), differ in their apparent agonist affinity. Here, we report the cloning of a third splice variant of RdL(ac), RDLad. Two-electrode voltage clamp electrophysiology was used to investigate agonistpharmacology of this expressed subunit following cRNA injection into Xenopus laevis oocytes. The EC50 values for GABA and its analogues isoguvacine, muscimol, isonipecotic acid and 3-amino sulphonic acid on the RDLad homomcric receptor differed from those previously described fur RDLac and DRC17-1-2 receptors. In addition to providing a possible physiological role for thealternative splicing of Rdl, these data delineate a hitherto functionally unassigned region of the N-terminal domain of GABA receptor subunits, whichaffects agonist potency and aligns closely with known determinants of potency in nicotinic acetylcholine receptors. Thus, using expression in Xenopus oocytes, we have demonstrated differences in agonist potency for the neurotransmitter GABA (and four analogues) between splice variant products of the Drosophila melanogaster Rdl gene encoding homomer-forming GABA receptor subunits. (C) 2001 IBRO. Published by Elsevier Science Ltd. All rights reserved.

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Documento generato il 09/04/20 alle ore 06:57:25