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Titolo:
Characterization of Corn1 mice: Alteration of epithelial and stromal cell gene expression
Autore:
Wang, IJ; Kao, CWC; Liu, CY; Saika, S; Nishina, PM; Sundberg, JP; Smith, RS; Kao, WWY;
Indirizzi:
Univ Cincinnati, Dept Ophthalmol, Cincinnati, OH 45267 USA Univ Cincinnati Cincinnati OH USA 45267 thalmol, Cincinnati, OH 45267 USA Jackson Lab, Bar Harbor, ME 04609 USA Jackson Lab Bar Harbor ME USA 04609Jackson Lab, Bar Harbor, ME 04609 USA
Titolo Testata:
MOLECULAR VISION
fascicolo: 4, volume: 7, anno: 2001,
pagine: 20 - 26
SICI:
1090-0535(20010208)7:4<20:COCMAO>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
STRATIFIED SQUAMOUS EPITHELIA; CORNIFIED ENVELOPE; BARRIER FUNCTION; KERATINOCYTE; DIFFERENTIATION; GROWTH; LOCALIZATION; SURFACE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Kao, WWY Univ Cincinnati, Dept Ophthalmol, Eden & Bethesda Ave, Cincinnati, OH 45267 USA Univ Cincinnati Eden & Bethesda Ave Cincinnati OH USA 45267 7 USA
Citazione:
I.J. Wang et al., "Characterization of Corn1 mice: Alteration of epithelial and stromal cell gene expression", MOL VIS, 7(4), 2001, pp. 20-26

Abstract

PURPOSE: Corn1 is an autosomal recessive mutation characterized by cornealepithelial hyperplasia and stromal neovascularization. The aim of the present study is to examine the expression patterns of specific epithelial and stromal proteins in corn/corn1 mutant mice. METHODS: Immunohistochemistry with antibodies directed against keratins 1,4, 5, 12, and 14 as well as loricrin, filaggrin, and involucrin were performed in corn1/corn1 and wild type, A.By/SnJ strain, mice at 4 weeks of age. Western blot hybridization was performed to confirm the presence of involucrin in corneas. In situ and northern blot hybridization were used to evaluate the expression of keratin 12, lumican, and keratocan in these mice. RESULTS: In corn1/corn1 mice, focal areas of corneal epithelial hyperplasia alternate with epithelium with normal appearance. Both regions of normal and hyperplastic corneal epithelium were labeled by anti-keratin 12 antibodies through all corneal epithelial layers. The anti-keratin 14 antibody only labeled the basal cell layer in normal epithelial areas, whereas it labeled both basal and suprabasal cell layers in hyperplastic areas. In wild type mice, anti-keratin 12 antibodies labeled all corneal epithelial layers, whereas anti-keratin 14 labeled the basal corneal epithelial cells only. Positive staining by anti-involucrin antibody was demonstrated in the basal corneal epithelial layer of wild type mice and normal areas of corn1/corn1 mice. Similarly, as observed with anti-keratin 14 antibody, the anti-involucrin antibody labeled both basal and suprabasal cell layers of hyperplastic corneal epithelium of corn1/corn1 mice. Antibodies against keratin 1, keratin 4, loricrin, and fillagrin did not label the corneas of wild type mice orcorn1/corn1 mice. Northern hybridization indicated that the expressions ofkeratocan and lumican mRNA levels were up regulated in corn1/corn1 mice, but keratin 12 mRNA remained similar to that of the wild type mice. In situ hybridization revealed that the lumican mRNA was detected in epithelial andstromal cells of corn1/corn1 mice, whereas keratocan mRNA was only detected in stromal cells. CONCLUSIONS: Hyperproliferative epithelial cells of corn1/corn1 mice have increased levels of expression of keratin 14 and involucrin, but do not exhibit the phenotypical characteristics of cornification. These observations indicate that factors associated with the phenotypes of corn1/corn1 mice donot alter the cornea-type epithelial differentiation of keratin 12 expression, but cause aberrant expression of lumican by corneal epithelial cells.

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Documento generato il 05/12/20 alle ore 08:12:05