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Titolo:
Involvement of annexin V in the antiproliferative effect of GnRH agonist on cultured human uterine leiomyoma cells
Autore:
Yamamoto, H; Sato, H; Shibata, S; Murata, M; Fukuda, J; Tanaka, T;
Indirizzi:
Akita Univ, Sch Med, Dept Obstet & Gynecol, Akita 0108543, Japan Akita Univ Akita Japan 0108543 pt Obstet & Gynecol, Akita 0108543, Japan
Titolo Testata:
MOLECULAR HUMAN REPRODUCTION
fascicolo: 2, volume: 7, anno: 2001,
pagine: 169 - 173
SICI:
1360-9947(200102)7:2<169:IOAVIT>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
GONADOTROPIN-RELEASING-HORMONE; PROTEIN-KINASE-C; SMOOTH-MUSCLE CELLS; IN-VITRO; CARCINOMA-CELLS; OVARIAN-CANCER; EXPRESSION; INHIBITION; RECEPTOR; REDISTRIBUTION;
Keywords:
annexin V; GnRH agonist; GnRH receptor; protein kinase C; uterine leiomyoma;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Tanaka, T Akita Univ, Sch Med, Dept Obstet & Gynecol, 1-1-1 Hondo, Akita 0108543, Japan Akita Univ 1-1-1 Hondo Akita Japan 0108543 Akita 0108543, Japan
Citazione:
H. Yamamoto et al., "Involvement of annexin V in the antiproliferative effect of GnRH agonist on cultured human uterine leiomyoma cells", MOL HUM REP, 7(2), 2001, pp. 169-173

Abstract

The objective of this study was to elucidate the role of annexin V, an endogenous inhibitor of protein kinase C (PKC), with regard to the antiproliferative effect of gonadotrophin-releasing hormone (GnRH) agonist (buserelin)on cultured human uterine leiomyoma cells. Uterine leiomyoma tissue was collected from the surgical specimens of patients and cells from 37 specimens(15 cases) were cultured. For up to 96 h after the addition of buserelin to the cultured cells, a time-dependent antiproliferative effect was noted in the group to which 10(-5) mol/I buserelin was added. Both the intracellular concentration of annexin V and the expression of annexin V mRNA increased time-dependently with the addition of buserelin, The intracellular concentration of annexin V increased with the addition of PKC activator ( 12-O-tetradecanoylphorbor- 13-acetate; TPA) much as it did with the addition of buserelin, and the rise in the concentration caused by the addition of buserelin was completely attenuated by pretreatment with PKC inhibitor (calphostin C). Our findings suggest that buserelin inhibits cell proliferation in cultured human uterine leiomyoma cells accompanied with an increase in the intracellular concentration of annexin V, mediated, at least in part, by the activation of PKC.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 22:56:38