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Titolo:
Quantitative measurement of transcript levels throughout human preimplantation development: analysis of hypoxanthine phosphoribosyl transferase
Autore:
Taylor, DM; Handyside, AH; Ray, PF; Dibb, NJ; Winston, RML; Ao, A;
Indirizzi:
Hammersmith Hosp, Dept Reprod Sci & Med, Div Obstet & Gynaecol, Imperial Coll Sch Med, London W12 0NN, England Hammersmith Hosp London England W12 0NN Sch Med, London W12 0NN, England Univ Leeds, Sch Biol, Leeds LS2 9JT, W Yorkshire, England Univ Leeds Leeds W Yorkshire England LS2 9JT S2 9JT, W Yorkshire, England Hop Necker Enfants Malad, Ctr Genet, U393, Inst Necker, Paris, France Hop Necker Enfants Malad Paris France U393, Inst Necker, Paris, France McGill Univ, Royal Victoria Hosp, Dept Obstet Gynaecol & Human Genet, Montreal, PQ H3A 1A1, Canada McGill Univ Montreal PQ Canada H3A 1A1 enet, Montreal, PQ H3A 1A1, Canada
Titolo Testata:
MOLECULAR HUMAN REPRODUCTION
fascicolo: 2, volume: 7, anno: 2001,
pagine: 147 - 154
SICI:
1360-9947(200102)7:2<147:QMOTLT>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
MATERNAL MESSENGER-RNAS; INVITRO FERTILIZATION; OOCYTE MATURATION; FOLLICULAR-FLUID; MOUSE EMBRYOS; POLY(A)+ RNA; EXPRESSION; POLYADENYLATION; ADENYLATION; ADENOSINE;
Keywords:
HPRT; human preimplantation embryo; oocyte; quantification; RT-PCR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Taylor, DM Hammersmith Hosp, Dept Reprod Sci & Med, Div Obstet & Gynaecol,Imperial Coll Sch Med, Du Cane Rd, London W12 0NN, England Hammersmith Hosp Du Cane Rd London England W12 0NN NN, England
Citazione:
D.M. Taylor et al., "Quantitative measurement of transcript levels throughout human preimplantation development: analysis of hypoxanthine phosphoribosyl transferase", MOL HUM REP, 7(2), 2001, pp. 147-154

Abstract

We have developed a competitive reverse transcription-polymerase chain reaction (RT-PCR) sensitive enough to detect and quantify as little as 2-fold differences in gene expression in individual oocytes and embryos throughouthuman preimplantation development, This RT-PCR assay can be tailored for the examination of any specific gene and so will give a unique insight into human preimplantation development. This technique was used to quantify the level of hypoxanthine phosphoribosyl transferase (HPRT) expression during preimplantation development and to correlate this with embryo sex. The amount of HPRT transcripts present in the unfertilized oocyte was equivalent to 7.7 fg of competitor cDNA. At the 4-cell stage there is a significant drop (P = 0.0006) to similar to1.2 fg. There was no detectable difference in theHPRT levels between female and male embryos following 2 days of in-vitro culture, In contrast HPRT gene expression was higher in day 3 female embryosthan in males. This is the first study to quantify gene transcripts throughout each stage of human preimplantation development and it indicates that the accumulated HPRT transcripts present in the unfertilized human oocyte undergo extensive destruction following fertilization. This work also suggests that X-inactivation occurs beyond the 8-cell stage of human preimplantation development.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/20 alle ore 07:25:57