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Titolo:
Effect of different FSH isoforms on cyclic-AMP production by mouse cumulus-oocyte-complexes: a time course study
Autore:
Andersen, CY; Leonardsen, L; Ulloa-Aguirre, A; Barrios-De-Tomasi, J; Kristensen, KS; Byskov, AG;
Indirizzi:
Univ Copenhagen Hosp, Reprod Biol Lab, Juliane Marie Ctr Children Women & Reprod, DK-2100 Copenhagen, Denmark Univ Copenhagen Hosp Copenhagen Denmark DK-2100 2100 Copenhagen, Denmark Hosp Ginecobstet Luis Castelazo Ayala, Res Unit Reprod Med, Inst Mexicano Seguro Social, Mexico City, DF, Mexico Hosp Ginecobstet Luis Castelazo Ayala Mexico City DF Mexico , DF, Mexico
Titolo Testata:
MOLECULAR HUMAN REPRODUCTION
fascicolo: 2, volume: 7, anno: 2001,
pagine: 129 - 135
SICI:
1360-9947(200102)7:2<129:EODFIO>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
FOLLICLE-STIMULATING-HORMONE; IN-VITRO; BIOLOGICAL-ACTIVITY; MENSTRUAL-CYCLE; RECEPTOR; SERUM; CHARGE; CELLS; HETEROGENEITY; ANALOGS;
Keywords:
cAMP; cumulus-oocyte-complexes; FSH; isoforms;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Leonardsen, L Univ Copenhagen Hosp, Reprod Biol Lab, Juliane Marie Ctr Children Women & Reprod, Sect 5712,Blegdamsvej 9, DK-2100 Copenhagen, Denmark Univ Copenhagen Hosp Sect 5712,Blegdamsvej 9 Copenhagen Denmark DK-2100
Citazione:
C.Y. Andersen et al., "Effect of different FSH isoforms on cyclic-AMP production by mouse cumulus-oocyte-complexes: a time course study", MOL HUM REP, 7(2), 2001, pp. 129-135

Abstract

The ability of different isoforms of follicle stimulating hormone (FSH) toinduce accumulation of cAMP in cultured mouse cumulus-oocyte-complexes (COC) was evaluated in a time course study. Using isoform fractions representing less acidic (pI 6,43-5.69), mid-acidic (pI 5.62-4.96) and acidic (pI 4.69-3.75) isoforms, the accumulation of cAMP was monitored after an exposure time of 0, 5, 10, 15, 30, 60, 120 and 180 min. In addition, cAMP productionwas monitored for 0, 5, 10, 15 and 30 min following a 5 min exposure to FSH isoform fractions, Based on FSH measurements using radioimmunoassays, theless and mid-acidic isoforms caused almost twice as much cAMP to be accumulated than the acidic isoform fraction, thereby confirming an enhanced biological activity of FSH isoforms with a isoelectric point (pI) of >5,0, For all isoform fractions, maximal accumulation of cAMP was achieved after 30 min of exposure, after which the production declined to background levels. After a 5 min exposure to isoform fractions, levels of cAMP were significantly higher in the less acidic isoform fractions, but after isoform removal, the decline in cAMP production to background levels followed a similar timecourse. The results demonstrate that FSH isoforms with a pi of >5.0 induced significant biological responses within a period of 30 min and that prolonged exposure caused attenuated signal transduction. The present results, set in the context of the pulsatile characteristics of FSH release from the pituitary and the reported half-life of less acidic isoforms of similar to 35 min, make it conceivable that isoforms with a pI >5.0 actually possess important physiological functions during the periovulatory period.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 02:23:18