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Titolo:
Alteration of cell growth and morphology by overexpression of transforminggrowth factor beta type II receptor in human lung adenocarcinoma cells
Autore:
Kim, TK; Mo, EK; Yoo, CG; Lee, CT; Han, SK; Shim, YS; Kim, YW;
Indirizzi:
Seoul Natl Univ, SNUMRC, Coll Med, Dept Internal Med, Seoul 110744, South Korea Seoul Natl Univ Seoul South Korea 110744 Med, Seoul 110744, South Korea Seoul Natl Univ, SNUMRC, Lung Inst, Seoul 110744, South Korea Seoul Natl Univ Seoul South Korea 110744 Inst, Seoul 110744, South Korea Seoul Natl Univ Hosp, Clin Res Inst, Seoul 110744, South Korea Seoul Natl Univ Hosp Seoul South Korea 110744 Seoul 110744, South Korea Hallym Univ, Coll Med, Dept Internal Med, Seoul 134701, South Korea HallymUniv Seoul South Korea 134701 rnal Med, Seoul 134701, South Korea
Titolo Testata:
LUNG CANCER
fascicolo: 2-3, volume: 31, anno: 2001,
pagine: 181 - 191
SICI:
0169-5002(200102/03)31:2-3<181:AOCGAM>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN PROSTATE-CANCER; TGF-BETA; REDUCED LEVELS; MESSENGER-RNA; EXPRESSION; LINES; INHIBITION; MUTATION; GENE; SENSITIVITY;
Keywords:
TGF-beta; TGF-beta receptor; dominant-negative; carcinogenesis; Rb protein; cyclin A; lung cancer;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Kim, YW Seoul Natl Univ, SNUMRC, Coll Med, Dept Internal Med, Seoul 110744, South Korea Seoul Natl Univ Seoul South Korea 110744 oul 110744, South Korea
Citazione:
T.K. Kim et al., "Alteration of cell growth and morphology by overexpression of transforminggrowth factor beta type II receptor in human lung adenocarcinoma cells", LUNG CANC, 31(2-3), 2001, pp. 181-191

Abstract

TGF-beta is a potent inhibitory regulator of cell growth. which is transduced through interaction between type I (RI) and type II (RII) receptors that form heteromeric kinase complexes. Abnormal expression of these receptorshas been identified in several human epithelial cancers and has been shownto be highly associated with resistance to TGF-beta (. ) In this study. we investigated the expression of RI and RII in 13 human non-small cell lung cancer cell lines (NSCLCs) and demonstrated decreased or loss of RII expression in five lung cancer cell lines, but not of RI. Of these cell lines, therole of RII in NCI-H358 adenocarcinoma. which lacks RII and is insensitiveto TGF-beta. was investigated by transducing this cell line with a recombinant retrovirus expressing full-length TGF-beta RI1. Stably transfected cells showed significant increase in RII mRNA and protein expression. These cells responded to exogenous TGF-beta1 with suppressed proliferation in a dose-dependent manner and G1 arrest accompanied by morphological change distinct from control cells. We also investigated whether overexpression of dominant-negative RII (dnRII) in NCI-H441 adenocarcinoma. which is sensitive butexpresses low levels of RII. could block signaling through the receptor complex. The overexpression of this kinase-domain-truncated RII by expressingthe retroviral dnRII construct led to loss of the ability to respond to TGF-beta1 and an exhibition of uncontrolled growth. These results suggest a close association between the loss of the expression of wild-type TGF-beta RII and carcinogenesis in human lune cancer cells. (C) 2001 Elsevier ScienceIreland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 08:53:00