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Titolo:
GLI-2 modulates retroviral gene expression
Autore:
Smith, MJ; Gitlin, SD; Browning, CM; Lane, BR; Clark, NM; Shah, N; Rainier, S; Markovitz, DM;
Indirizzi:
Univ Michigan, Med Ctr, Dept Internal Med, Ann Arbor, MI 48109 USA Univ Michigan Ann Arbor MI USA 48109 nternal Med, Ann Arbor, MI 48109 USA Univ Michigan, Med Ctr, Dept Immunol & Microbiol, Ann Arbor, MI 48109 USA Univ Michigan Ann Arbor MI USA 48109 & Microbiol, Ann Arbor, MI 48109 USA Univ Michigan, Med Ctr, Program Cellular & Mol Biol, Ann Arbor, MI 48109 USA Univ Michigan Ann Arbor MI USA 48109 & Mol Biol, Ann Arbor, MI 48109 USA
Titolo Testata:
JOURNAL OF VIROLOGY
fascicolo: 5, volume: 75, anno: 2001,
pagine: 2301 - 2313
SICI:
0022-538X(200103)75:5<2301:GMRGE>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; T-CELL LEUKEMIA; LONG TERMINAL REPEAT; CUBITUS-INTERRUPTUS PROTEIN; NF-KAPPA-B; ZINC-FINGER PROTEINS; DNA-BINDING PROTEIN; TRANSCRIPTION FACTOR; TYPE-2 ENHANCER; SONIC HEDGEHOG;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
98
Recensione:
Indirizzi per estratti:
Indirizzo: Markovitz, DM 5220 MSRB 3,1150 W Med Ctr Dr, Ann Arbor, MI 48109 USA 5220MSRB 3,1150 W Med Ctr Dr Ann Arbor MI USA 48109 09 USA
Citazione:
M.J. Smith et al., "GLI-2 modulates retroviral gene expression", J VIROLOGY, 75(5), 2001, pp. 2301-2313

Abstract

GLI proteins are involved in the development of mice, humans, zebrafish, Caenorhabditis elegans, Xenopus, and Drosophila. While these zinc finger-containing proteins bind to TO-rich promoter elements and are known to regulate gene expression in C. elegans and Drosophila, mechanistic understanding of how regulation is mediated through naturally occurring transcriptional promoters is lacking. One isoform of human GLI-2 appears to be identical to afactor previously called Tax helper protein (THP), thus named due to its ability to interact with a TO-rich element in the human T-lymphotropic virustype 1 (HTLV-1) enhancer thought to mediate transcriptional stimulation bythe Tax protein of HTLV-1. We now demonstrate that, working through its TO-rich binding site and adjacent elements, GLI-2/THP actually suppresses gene expression driven by the HTLV-1 promoter. GLI-2/THP has no effect on the HTLV-2 promoter, activates expression from the promoters of human immunodeficiency virus types 1 and (HIV-1 and -2), and stimulates HIV-1 replication. Both effective suppression and activation of gene expression and viral replication require the first of the five zinc fingers, which is not necessaryfor DNA binding, to be intact. Thus, not only can GLI-2/THP either activate or suppress gene expression, depending on the promoter, but the same domain (first zinc finger) mediates both effects. These findings suggest a rolefor GLI-2 in retroviral gene regulation and shed further light on the mechanisms by which GLI proteins regulate naturally occurring promoters.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/09/20 alle ore 02:21:18