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Titolo:
Storage of bovine viral diarrhoea virus samples on filter paper and detection of viral RNA by a RT-PCR method
Autore:
Vilcek, S; Strojny, L; Durkovic, B; Rossmanith, W; Paton, D;
Indirizzi:
Univ Vet Med, Kosice 04181, Slovakia Univ Vet Med Kosice Slovakia 04181Univ Vet Med, Kosice 04181, Slovakia Vet Med Res Inst, Kosice 04000, Slovakia Vet Med Res Inst Kosice Slovakia 04000 Res Inst, Kosice 04000, Slovakia Govt Lower Austria, Vet Off, A-3109 St Polten, Austria Govt Lower AustriaSt Polten Austria A-3109 f, A-3109 St Polten, Austria Vet Lab Agcy Weybridge, Weybridge KT15 3NB, Surrey, England Vet Lab Agcy Weybridge Weybridge Surrey England KT15 3NB Surrey, England
Titolo Testata:
JOURNAL OF VIROLOGICAL METHODS
fascicolo: 1, volume: 92, anno: 2001,
pagine: 19 - 22
SICI:
0166-0934(200103)92:1<19:SOBVDV>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; BLOOD; PESTIVIRUSES; CATTLE;
Keywords:
bovine viral diarrhoea virus; BVDV; filter; RT-PCR; detection;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
13
Recensione:
Indirizzi per estratti:
Indirizzo: Vilcek, S Univ Vet Med, Komenskeho 73, Kosice 04181, Slovakia Univ Vet MedKomenskeho 73 Kosice Slovakia 04181 4181, Slovakia
Citazione:
S. Vilcek et al., "Storage of bovine viral diarrhoea virus samples on filter paper and detection of viral RNA by a RT-PCR method", J VIROL MET, 92(1), 2001, pp. 19-22

Abstract

Of four solid carriers tested, Whatman paper No 1 was the best for storingblood and serum samples for the diagnosis of bovine viral diarrhoea (BVD) by means of viral RNA detection. The filter papers were impregnated with 10mul of blood or serum, followed by air drying. Samples collected in this way from persistently infected animals had lost infectivity within a few days, but viral RNA could still be detected by RT-PCR for up to 6 months. Wheninvestigated by RT-PCR, 12 blood and 10 serum samples selected at random from animals persistently infected with BVD virus showed the same results whether samples had been spotted onto filters or examined directly from the liquid state. The filters spotted with blood or serum are convenient for storage and transport of samples to a diagnostic laboratory without the need for cooling. Sequencing of amplified RNA can be used subsequently for genetic typing. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 11:36:40