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Titolo:
Marking hypoxia in rat prostate carcinomas with beta-D-[125I]azomycin galactopyranoside and [Tc-99m]HL-91: Correlation with microelectrode measurements
Autore:
Iyer, RV; Haynes, PT; Schneider, RF; Movsas, B; Chapman, JD;
Indirizzi:
Fox Chase Canc Ctr, Dept Radiat Oncol, Philadelphia, PA 19111 USA Fox Chase Canc Ctr Philadelphia PA USA 19111 , Philadelphia, PA 19111 USA
Titolo Testata:
JOURNAL OF NUCLEAR MEDICINE
fascicolo: 2, volume: 42, anno: 2001,
pagine: 337 - 344
SICI:
0161-5505(200102)42:2<337:MHIRPC>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
PREDICTS RADIATION RESPONSE; TUMOR HYPOXIA; OXYGENATION; MARKER; CANCER; CELLS; SURVIVAL; THERAPY; ARABINOSIDE; TENSION;
Keywords:
halothane; anesthesia; Eppendorf Po-2 microelectrode; HL-91; beta-D-IAZGP; hypoxic markers; tumor oxygenation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Chapman, JD Fox Chase Canc Ctr, Dept Radiat Oncol, 7701 Burholme Ave, Philadelphia, PA19111 USA Fox Chase Canc Ctr 7701 Burholme Ave Philadelphia PA USA 19111
Citazione:
R.V. Iyer et al., "Marking hypoxia in rat prostate carcinomas with beta-D-[125I]azomycin galactopyranoside and [Tc-99m]HL-91: Correlation with microelectrode measurements", J NUCL MED, 42(2), 2001, pp. 337-344

Abstract

The purpose of this study was to determine, with a rodent tumor model, if microelectrode measurements of unmodulated tumor oxygenation predict for the avidity of hypoxic markers to tumor tissue. Methods: The rapidly growing,anaplastic variant of the Dunning rat prostate carcinoma cell line (R3327-AT) was implanted subcutaneously on the upper backs of Fischer X Copenhagenrats. Approximately 100 measurements of Po-2 were obtained from tumors of 5-10 g in animals that were restrained and then subjected to different anesthetic procedures. Values of median Po-2 (in mm Hg) and percentage of measurements (5 mm Hg obtained from individual tumors were used to define tumor oxygenation status. The radiodiagnostic hypoxic markets P-D-iodinated azomycin galactopyranoside (IAZGP) and [Tc-99m]HL-91 were simultaneously administered to 26 animals whose tumor oxygen levels had been measured. Six hours after marker administration, the animals were killed; tumor, blood, and muscle tissues were sampled; and percentage injected dose per gram (%ID/g*), tumor/blood ratio (T/B), and tumor/muscle ratio (T/M) parameters were determined. Parameters of marker avidity to individual tumors were linearly correlated with microelectrode measurements of tumor oxygenation to determine the significance of inverse associations. Results: The median Po-2 Of 41 tumors varied from 2.0 to 20.9 mm Hg, with an average value of 7.5 +/- 1.4 mm Hg. Six tumors had unusually high values; that is, >10 mm Hg, and when thesewere excluded from the analysis, the average median Po-2 of the remaining 35 was 4.3 +/- 0.7 mm Hg. When electrode measurements of tumor oxygenation were obtained under conditions of halothane anesthesia with the animals breathing O-2, carbogen, or air, median Po-2 values increased significantly (P= 0.001). When animals were deeply anesthetized by intraperitoneal injection of ketamine-xylazine, median Po-2 values were not significantly different (P = 0.13) from those obtained while the animals were restrained and breathing air. There was no inverse correlation of significance between the electrode measurements of median Po-2 and the avidity of beta -D-IAZGP nor [99mTc]HL-91 in this tumor model. The range of median Po-2 values in these tumors was at least 3 mm Hg, and the range of hypoxic marker avidity was less than twofold. Conclusion: These data demonstrate that microelectrode measurements of rat tumor oxygenation did not correlate with the avidity of the two hypoxic markers, at least in this tumor model. The larger dynamic range of tumor oxygen measurements obtained with microelectrodes might be biased to low values by their necrotic fractions, the zones within solid tumors that contain dead cells and debris that will not be labeled by bioreducible hypoxic markers. Hypoxic marker avidity to individual tumors will have to bevalidated by other assays that can predict for their radiosensitivity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 04:14:12