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Titolo:
Nano-electrospray ionization time-of-flight mass spectrometry of gangliosides from human brain tissue
Autore:
Metelmann, W; Vukelic, Z; Peter-Katalinic, J;
Indirizzi:
Univ Munster, Inst Med Phys & Biophys, Lab Biomed Anal, D-48149 Munster, Germany Univ Munster Munster Germany D-48149 omed Anal, D-48149 Munster, Germany
Titolo Testata:
JOURNAL OF MASS SPECTROMETRY
fascicolo: 1, volume: 36, anno: 2001,
pagine: 21 - 29
SICI:
1076-5174(200101)36:1<21:NITMSO>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
NERVOUS-SYSTEM; CELL-LINE; GLYCOSPHINGOLIPIDS; BINDING;
Keywords:
human brain gangliosides; GM1; GD1 and GT1; nano-electrospray ionization quadrupole time-of-flight mass spectrometry; low-energy collision-induced dissociation; native and permethylated gangliosides;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Peter-Katalinic, J Univ Munster, Inst Med Phys & Biophys, Lab Biomed Anal,Robert Koch Str 31, D-48149 Munster, Germany Univ Munster Robert Koch Str 31 Munster Germany D-48149
Citazione:
W. Metelmann et al., "Nano-electrospray ionization time-of-flight mass spectrometry of gangliosides from human brain tissue", J MASS SPEC, 36(1), 2001, pp. 21-29

Abstract

A general approach for the detection and structural elucidation of brain ganglioside species GM1, GD1 and GT1 by nano-electrospray ionization quadrupole time-of-flight (nanoESI-QTOF) mass spectrometry (MS), using combined data from MS and MS/MS analysis of isolated native ganglioside fractions in negative ion mode and their permethylated counterparts in the positive ion mode is presented. This approach was designed to detect and sequence gangliosides present in preparatively isolated ganglioside fractions from pathological brain samples available in only very limited amounts. In these fractions mixtures of homologue and isobaric structures are present, depending on the ceramide composition and the position of the sialic acid attachment site. The interpretation of data for the entire sequence, derived from A, B, Cand Y ions by nanoESI-QTOFMS/MS in the negative ion mode of native fractions, can be compromized by ions arising from double and triple internal cleavages. To distinguish between isobaric carbohydrate structures in gangliosides, such as monosialogangliosides GM1a and GM1b, disialogangliosides GD1a,GD1b and GD1e or trisialogangliosides GT1b, GT1c and GT1d, the samples were analysed after permethylation in the positive ion nanoESI-QTOFMS/MS mode,providing set of data, which allows a clear distinction for assignment of outer and inner fragment ions according to their m/z values. The fragmentation patterns from native gangliosides obtained by low-energy collision induced dissociation (CID) by nanoESI-QTOF show common behaviour and follow inherent rules. The combined set of data from the negative and positive ion mode low-energy CID can serve for the detection of structural isomers in mixtures, and to trace new, not previously detected, components. Copyright (C) 2001 John Wiley & Sons, Ltd.

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Documento generato il 05/04/20 alle ore 06:19:38