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Titolo:
CpG-containing oligodeoxynucleotides induce TNF-alpha and IL-6 production but not degranulation from murine bone marrow-derived mast cells
Autore:
Zhu, FG; Marshall, JS;
Indirizzi:
Dalhousie Univ, Dept Microbiol & Immunol, Halifax, NS B3H 4H7, Canada Dalhousie Univ Halifax NS Canada B3H 4H7 nol, Halifax, NS B3H 4H7, Canada Dalhousie Univ, Dept Pathol, Halifax, NS B3H 4H7, Canada Dalhousie Univ Halifax NS Canada B3H 4H7 hol, Halifax, NS B3H 4H7, Canada
Titolo Testata:
JOURNAL OF LEUKOCYTE BIOLOGY
fascicolo: 2, volume: 69, anno: 2001,
pagine: 253 - 262
SICI:
0741-5400(200102)69:2<253:COITAI>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
NECROSIS-FACTOR-ALPHA; BACTERIAL-DNA; DENDRITIC CELLS; SYNTHETIC OLIGONUCLEOTIDES; IMMUNOSTIMULATORY DNA; HISTAMINE-RELEASE; INTERFERON-GAMMA; B-CELL; ACTIVATION; MOTIFS;
Keywords:
DNA; cytokines; inflammation; bacteria;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Marshall, JS Dalhousie Univ, Dept Microbiol & Immunol, Sir Charles Tupper Med Bldg, Halifax, NS B3H 4H7, Canada Dalhousie Univ Sir Charles Tupper MedBldg Halifax NS Canada B3H 4H7
Citazione:
F.G. Zhu e J.S. Marshall, "CpG-containing oligodeoxynucleotides induce TNF-alpha and IL-6 production but not degranulation from murine bone marrow-derived mast cells", J LEUK BIOL, 69(2), 2001, pp. 253-262

Abstract

Mast cells are sentinel cells critical to the initiation of innate immune and inflammatory responses, particularly at mucosal surfaces, To fulfill this function they can be activated by several pathogen-associated stimuli toproduce cytokines with or without concurrent degranulation, We examined the ability of immunostimulatory DNA sequences including CpG motifs, which are found in increased quantities in bacterial DNA, to activate mouse bone marrow-derived mast cells (mBMMC). Mast cells were treated with a range of doses of CpG-containing oligodeoxynucleotides or control oligodeoxynucleotides without CpG Tvithin their sequence. There was a dose-dependent increase in the production of both interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-alpha) by mast cells treated with the CpG-containing oligodeoxynucleotides. The cytokine levels induced were directly related to the number ofCpG within a given length of sequence, Treatment with oligonucleotides containing 3CpG induced an eightfold increase in TNF production over control incubated mast cells, Other cytokines, including granulocyte-macrophage colony-stimulating factor, IL-4, iuterferon-gamma, and IL-12 were not induced by oligonucleotide treatment, Neither CpG containing oligodeoxynucleotides nor control oligodeoxynucleotides induced degranulation of mast cells, Bacterial DNA from Escherichia coli also induced IL-6 from mBMMC but neither calf thymus DNA nor methylase-treated E. coli DNA had such an effect, Examination of the uptake of Texas red-labeled CpG and non-CpG-containing oligodeoxynucleotides revealed that they were both similarly taken up by the mBMMC, These results have important implications for the mechanism by which mast cells respond to bacteria and for the potential role of mast cells in DNA vaccination.

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Documento generato il 03/04/20 alle ore 07:15:51