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Titolo:
Correlation between apoptosis macroarray gene expression profiling and histopathological lymph node lesions
Autore:
Dales, JP; Plumas, J; Palmerini, F; Devilard, E; Defrance, T; Lajmanovich, A; Pradel, V; Birg, F; Xerri, L;
Indirizzi:
Inst J Paoli I Calmettes, Dept Pathol, IFR 57, F-13273 Marseille 9, FranceInst J Paoli I Calmettes Marseille France 9 F-13273 Marseille 9, France Univ Mediterranee, F-13000 Marseille, France Univ Mediterranee MarseilleFrance F-13000 ee, F-13000 Marseille, France ETS Isere Savoie, Dept Immunol, F-38000 Grenoble, France ETS Isere SavoieGrenoble France F-38000 munol, F-38000 Grenoble, France Unite UPRES 2021, Res Grp Lymphomas, F-38000 Grenoble, France Unite UPRES 2021 Grenoble France F-38000 homas, F-38000 Grenoble, France INSERM, U404, F-65000 Lyon, France INSERM Lyon France F-65000INSERM, U404, F-65000 Lyon, France Hop St Marguerite, Dept Biostat, F-13000 Marseille, France Hop St Marguerite Marseille France F-13000 at, F-13000 Marseille, France
Titolo Testata:
JOURNAL OF CLINICAL PATHOLOGY-MOLECULAR PATHOLOGY
fascicolo: 1, volume: 54, anno: 2001,
pagine: 17 - 23
SICI:
1366-8714(200102)54:1<17:CBAMGE>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
FAS-MEDIATED APOPTOSIS; NON-HODGKINS-LYMPHOMA; CENTER B-CELLS; PROTEASE; DEATH; CLASSIFICATION; CASPASE-3; RESISTANT; TISSUES; COMPLEX;
Keywords:
macroarrays; apoptosis; gene profiling; non-Hodgkin's lymphomas; caspases; immunohistochemistry;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Xerri, L Inst J Paoli I Calmettes, Dept Pathol, IFR 57, 232 Blvd St Marguerite,BP 156, F-13273 Marseille 9, France Inst J Paoli I Calmettes 232 Blvd St Marguerite,BP 156 Marseille France 9
Citazione:
J.P. Dales et al., "Correlation between apoptosis macroarray gene expression profiling and histopathological lymph node lesions", J CL PATH-M, 54(1), 2001, pp. 17-23

Abstract

Ai,Aims-Microarray technology has recently led to the identification of molecular prognostic subgroups in non-Hodgkin's lymphomas. To determine the usefulness of ready made macroarrays as routine diagnostic tools in haematopathology, lymph node biopsies were analysed using a cDNA macroarray containing genes involved in apoptosis, including caspases. Methods-Nine biopsy specimens were analysed using total frozen tissues: four samples of B cell follicular lymphoma, two of B cell diffuse large cell lymphoma, and three of non-neoplastic lymph nodes from benign lymphadenitis. Nine cell populations were sorted from fresh tissues: malignant B cells from two patients with follicular lymphoma and two with diffuse large cell lymphoma, reactive B cells from two benign lymph nodes, reactive T cells from one benign lymph node, and virgin (mantle zone) B cells and germinal centre B cells from benign tonsils. Immunohistochemistry (IHC) on paraffin wax sections was performed for the localisation of caspases 2, 3, 4, 7, 8, and 9. Results-In the clustered array data, sorted cells from samples sharing common histological lesions were grouped together, whereas the array/histologycorrelation was less satisfactory for tissues. The expression profiles of both the array and IHC methods correlated for most caspases and samples. Conclusions-Variations in array profiles of sorted cell populations can beassociated with specific histological features, suggesting a possible diagnostic application of ready made apoptosis macroarrays in haematopathology.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/09/20 alle ore 00:58:00