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Titolo:
Determination of monobromobimane derivatives of phenylmercapturic and benzylmercapturic acids in urine by high-performance liquid chromatography and fluorimetry
Autore:
Buratti, M; Brambilla, G; Fustinoni, S; Pellegrino, O; Pulviremti, S; Colombi, A;
Indirizzi:
Ist Clin Perfezionamento, Lab Tossicol Profess, I-20122 Milan, Italy Ist Clin Perfezionamento Milan Italy I-20122 ofess, I-20122 Milan, Italy Univ Milan, Fac Med, Dept Occupat Hlth, S Paolo Hosp, I-20142 Milan, ItalyUniv Milan Milan Italy I-20142 Hlth, S Paolo Hosp, I-20142 Milan, Italy
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 2, volume: 751, anno: 2001,
pagine: 305 - 313
SICI:
1387-2273(20010225)751:2<305:DOMDOP>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
OCCUPATIONAL EXPOSURE; MERCAPTURIC ACIDS; BENZENE EXPOSURE; METABOLITES; BIOMARKER; MECHANICS; BLOOD;
Keywords:
exposure biomarkers; tobacco smoking; phenylmercapturic acid; benzylmercapturic acid; toluene; benzene;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Buratti, M Ist Clin Perfezionamento, Lab Tossicol Profess, Via Commenda 10, I-20122 Milan, Italy Ist Clin Perfezionamento Via Commenda 10 Milan Italy I-20122 y
Citazione:
M. Buratti et al., "Determination of monobromobimane derivatives of phenylmercapturic and benzylmercapturic acids in urine by high-performance liquid chromatography and fluorimetry", J CHROMAT B, 751(2), 2001, pp. 305-313

Abstract

A method was developed for the determination in human urine of S-phenylmercapturic (PMA) and S-benzylmercapturic (BMA) acids, metabolites respectively of benzene and toluene. PMA and BMA were determined, after alkaline hydrolysis, to give respectively thiophenol and benzylmercaptan, and coupling ofthe thiol-containing compounds with monobromobimane (MB), by reversed-phase HPLC on a diphenyl-silica bonded cartridge (100x4.6 mm I.D., 5 mum particle size) with fluorimetric detection. Wavelengths for excitation and emission were 375 and 480 nm, respectively. The recovery of PMA and BMA from spiked urines was >90% in the 10-500 mug/l range; the quantification limits were respectively 1 and 0.5 mug/l; day-to-day precision at 42 mug/l was C.V. <7%. The suitability of the proposed procedure for the biological monitoringof exposure to low-level airborne concentrations of benzene and toluene, was evaluated by analyzing the urinary excretion of PMA and BMA in subjects exposed to different sources of aromatic hydrocarbons, namely occupationally-unexposed referents (non-smokers, n=15; moderate smokers, n=8; mean number of cigarettes smoked per-day=17 cig/day) and non-smoker workers occupationally exposed to toluene in maintenance operations of rotogravure machines (non-smokers, n=17). Among referents, non-smokers showed values of PMA ranging from <1 to 4.6 mug/l and BMA from 1.0 to 10.4 mug/l; in smokers, PMA values ranging from 1.2 to 6.7 mug/l and BMA from 9.3 to 39.9 mug/l, were observed. In occupationally exposed non-smoker subjects, BMA median excretion value (23.6 mug/l) was higher than in non-smoker referents (3.5 mug/l) (P<0.001) and individual BMA values (y, <mu>g/l) were associated and increased with airborne toluene concentration Gs, mg/m(3)) according to the equation y=6.5+0.65x (r=0.69, P<0.01, n=17). The proposed analytical method appears to be a sensitive and specific tool for biological monitoring of low-level exposure to benzene and toluene mixtures in occupational and environmental toxicology laboratory. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/20 alle ore 22:12:37