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Titolo:
Simple method for clinical determination of 13 carotenoids in human plasmausing an isocratic high-performance liquid chromatographic method
Autore:
Lyan, B; Azais-Braesco, V; Cardinault, N; Tyssandier, V; Borel, P; Alexandre-Gouabau, MC; Grolier, P;
Indirizzi:
INRA, CRNH Ctr Clermont Ferrand Theix, Unite Malad Metab & Micronutriments, F-63122 St Genes Champanelle, France INRA St Genes Champanelle France F-63122 22 St Genes Champanelle, France
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 2, volume: 751, anno: 2001,
pagine: 297 - 303
SICI:
1387-2273(20010225)751:2<297:SMFCDO>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
BETA-CAROTENE; ALPHA-TOCOPHEROL; PHASE HPLC; VITAMIN-A; RETINOL; ISOMERS; SERUM; QUANTIFICATION; ZEAXANTHIN; LYCOPENE;
Keywords:
carotenoids;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Grolier, P INRA, CRNH Ctr Clermont Ferrand Theix, Unite Malad Metab & Micronutriments, F-63122 St Genes Champanelle, France INRA St Genes Champanelle France F-63122 Champanelle, France
Citazione:
B. Lyan et al., "Simple method for clinical determination of 13 carotenoids in human plasmausing an isocratic high-performance liquid chromatographic method", J CHROMAT B, 751(2), 2001, pp. 297-303

Abstract

We report a reversed-phase high-performance liquid chromatography method which resolves 13 identified carotenoids and nine unknown carotenoids from human plasma. A Nucleosil C-18 column and a Vydac C-18 column in series are used with an isocratic solvent system of acetonitrile-methanol containing 50 mM acetate ammonium-dichloromethane - water (70:15:10:5, v/v/v/v) as mobile phase at a flow-rate of 2 ml/min. The intra-day (4.5-8.3%) and inter-day(1.3-12.7%) coefficients of variation are suitable for routine clinical determinations. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/20 alle ore 22:15:58