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Titolo:
Modified method for the determination of capillary electrophoresis nitric oxide-correlated nitrate in tissue homogenates
Autore:
Tuncel, M; Dogrukol-Ak, D; Erkasap, N;
Indirizzi:
Univ Anadolu, Fac Pharm, Dept Analyt Chem, TR-26470 Eskisehir, Turkey UnivAnadolu Eskisehir Turkey TR-26470 Chem, TR-26470 Eskisehir, Turkey Univ Osmangazi, Fac Med, Dept Physiol, TR-26040 Eskisehir, Turkey Univ Osmangazi Eskisehir Turkey TR-26040 iol, TR-26040 Eskisehir, Turkey
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 2, volume: 751, anno: 2001,
pagine: 257 - 263
SICI:
1387-2273(20010225)751:2<257:MMFTDO>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
ZONE ELECTROPHORESIS; RELEASE; PLASMA;
Keywords:
capillary electrophoresis; nitric oxide-correlated nitrate; tissue homogenates;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Tuncel, M Univ Anadolu, Fac Pharm, Dept Analyt Chem, TR-26470 Eskisehir, Turkey Univ Anadolu Eskisehir Turkey TR-26470 26470 Eskisehir, Turkey
Citazione:
M. Tuncel et al., "Modified method for the determination of capillary electrophoresis nitric oxide-correlated nitrate in tissue homogenates", J CHROMAT B, 751(2), 2001, pp. 257-263

Abstract

A modified capillary electrophoretic method for the determination of nitric oxide correlated nitrate in several tissue homogenates is described in this study. The method was developed using a running buffer consisting of 200mM lithium chloride and 10 mM berate buffer at pH 8.5, in a fused-silica column total 82 cm, effective 43 cm length and 75 mum I.D. The signal was measured at 214 nm and controlled current of 200 muA (equivalent to 12.7 kV) was applied in the reversed polarity direction. The sample was injected by vacuum pressure 50 ms (25 nl). In these conditions, bromide as internal standard and nitrate appeared at 7.2 and 8.9 min, respectively. Whole validation procedures were applied and satisfactory results were obtained. The nitrate levels of the tissue homogenates of control and L-NAME applied (heart, brain, kidney, stomach, lung, testis and liver) were monitored by the present method and it was decided that the method is precise and accurate. (C) 2001 Elsevier Science B.V. All rights reserved.

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Documento generato il 02/06/20 alle ore 01:40:34