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Titolo:
Determination of high-energy phosphate compounds and inorganic phosphate by reversed-phase high-performance liquid chromatography: evaluation of myocardial metabolic status in aerobically perfused and hypoxic mouse heart
Autore:
Tomasetti, M; Graciotti, L; Fulgenzi, G; Granata, AL;
Indirizzi:
Univ Ancona, Fac Med, Inst Expt Pathol, I-60131 Ancona, Italy Univ AnconaAncona Italy I-60131 Inst Expt Pathol, I-60131 Ancona, Italy
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 2, volume: 751, anno: 2001,
pagine: 229 - 236
SICI:
1387-2273(20010225)751:2<229:DOHPCA>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
PHOSPHORYLATED METABOLITES; ADENINE-NUCLEOTIDES; RAT-LIVER; P-31 NMR; SEPARATION; ISCHEMIA; TISSUE; ATP;
Keywords:
inorganic phosphate; high-energy phosphate;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Tomasetti, M Univ Ancona, Fac Med, Inst Expt Pathol, Via Ranieri, I-60131 Ancona, Italy Univ Ancona Via Ranieri Ancona Italy I-60131 1 Ancona, Italy
Citazione:
M. Tomasetti et al., "Determination of high-energy phosphate compounds and inorganic phosphate by reversed-phase high-performance liquid chromatography: evaluation of myocardial metabolic status in aerobically perfused and hypoxic mouse heart", J CHROMAT B, 751(2), 2001, pp. 229-236

Abstract

The present paper describes a simple HPLC method designed for measuring high-energy phosphate (HEP) compounds in a single run and inorganic phosphate(P-i) in an other short run under the same HPLC conditions. Inorganic phosphate was estimated by using thymidine phosphorylase (EC 2.4.2.4) which catalyzes a reaction involving inorganic phosphate to produce 2-deoxyribose 1-phosphate and thymine. The thymine/P-i stoichiometry was 1. The method provides a reproducible instrument fur evaluating myocardial high-energy metabolism under physiological and pathological conditions. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/01/20 alle ore 07:15:30