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Titolo:
Development of a technology for automation and miniaturization of protein crystallization
Autore:
Mueller, U; Nyarsik, L; Horn, M; Rauth, H; Przewieslik, T; Saenger, W; Lehrach, H; Eickhoff, H;
Indirizzi:
Max Planck Inst Mol Genet, D-14195 Berlin, Germany Max Planck Inst Mol Genet Berlin Germany D-14195 D-14195 Berlin, Germany Free Univ Berlin, Inst Crystallog, D-14195 Berlin, Germany Free Univ Berlin Berlin Germany D-14195 stallog, D-14195 Berlin, Germany DHGP, Resource Ctr, Prot Struct Factory, D-14059 Berlin, Germany DHGP Berlin Germany D-14059 Prot Struct Factory, D-14059 Berlin, Germany
Titolo Testata:
JOURNAL OF BIOTECHNOLOGY
fascicolo: 1, volume: 85, anno: 2001,
pagine: 7 - 14
SICI:
0168-1656(20010123)85:1<7:DOATFA>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENE-EXPRESSION;
Keywords:
protein crystallization; microplate; miniaturization; automation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
7
Recensione:
Indirizzi per estratti:
Indirizzo: Eickhoff, H Max Planck Inst Mol Genet, Ihnestr 73, D-14195 Berlin, GermanyMax Planck Inst Mol Genet Ihnestr 73 Berlin Germany D-14195 y
Citazione:
U. Mueller et al., "Development of a technology for automation and miniaturization of protein crystallization", J BIOTECH, 85(1), 2001, pp. 7-14

Abstract

The usage of standard 96 well microplates for the screening of crystallization conditions of recombinant proteins offers several advantages when compared to commonly used crystallization plate formats. The adoption of robotic technology for plate and glass slide preparation within a 'hanging drop' vapour diffusion crystallization experiment enables to work with an increased throughput at reduced costs. In addition to commercial pipetting deviceswith a 96-channel aspirator/dispenser, solenoid ink-jet technology was applied to form 250 nl droplets with a diameter of similar to1 mm. This allowsminiaturization of crystallization screening set-ups with an estimated ten-fold cost reduction when compared to commonly used 24 well plates. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 09:41:57