Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Functional elements of the strong psbAI promoter of Synechococcus elongatus PCC 7942
Autore:
Nair, U; Thomas, C; Golden, SS;
Indirizzi:
Texas A&M Univ, Dept Biol, College Stn, TX 77843 USA Texas A&M Univ College Stn TX USA 77843 t Biol, College Stn, TX 77843 USA
Titolo Testata:
JOURNAL OF BACTERIOLOGY
fascicolo: 5, volume: 183, anno: 2001,
pagine: 1740 - 1747
SICI:
0021-9193(200103)183:5<1740:FEOTSP>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
SP STRAIN PCC-7942; CIRCADIAN GENE-EXPRESSION; GROUP-2 SIGMA-FACTORS; HIGH LIGHT-INTENSITY; ANACYSTIS-NIDULANS; SP STRAIN-PCC-7942; RNA-POLYMERASE; CYANOBACTERIA; SEQUENCES; TRANSCRIPTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Golden, SS Texas A&M Univ, Dept Biol, 3258 TAMU, College Stn, TX 77843 USATexas A&M Univ 3258 TAMU College Stn TX USA 77843 TX 77843 USA
Citazione:
U. Nair et al., "Functional elements of the strong psbAI promoter of Synechococcus elongatus PCC 7942", J BACT, 183(5), 2001, pp. 1740-1747

Abstract

The psbAI gene of the cyanobacterium Synechococcus elongatus PCC 7942 is one of three psbA genes that encode a critical photosystem IT reaction center protein, D1, Regulation of the gene family in response to changes in the light environment is complex, occurs at transcriptional and posttranscriptional levels, and results in an interchange of two different forms of D1 in the membrane. Expression of psbAI is downregulated under high-intensity light (high light) in contrast to induction of the other two family members. We show that, in addition to a known accelerated degradation of the psbAI message, promoter activity decreases upon exposure to high light. Unlike the other psbA genes, additional sequences upstream of the psbAI -35 element are required for expression. Mutagenizing the atypical psbAI - 10 element from TCTCCT to TATAAT increased the magnitude of expression from both psbAI::lacZ and psbAI::luxAB fusions but did not affect downregulation under high light, Inactivation of group 2 sigma factor genes rpoD2 and sigC, in both wild-type and - 10-element mutagenized backgrounds, resulted in elevated psbAI::luxAB expression but did not alter the response to high light. The results are consistent with redundancy of promoter recognition among cyanobacterial group 2 sigma factors. Electrophoretic mobility shift assays showed that the DNA sequence corresponding to the untranslated leader of the psbAI message binds one or more proteins from an S. elongatus extract. The corresponding region of psbAI efficiently competed for this binding activity, suggesting a shared regulatory factor among these disparately regulated genes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/10/20 alle ore 11:06:02