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Titolo:
Isolation and characterization of a zebrafish homologue of the cone rod homeobox gene
Autore:
Liu, YH; Shen, YC; Rest, JS; Raymond, PA; Zack, DJ;
Indirizzi:
Johns Hopkins Univ, Sch Med, Dept Ophthalmol, Baltimore, MD 21287 USA Johns Hopkins Univ Baltimore MD USA 21287 halmol, Baltimore, MD 21287 USA Johns Hopkins Univ, Sch Med, Dept Neurosci, Baltimore, MD 21287 USA Johns Hopkins Univ Baltimore MD USA 21287 urosci, Baltimore, MD 21287 USA Johns Hopkins Univ, Sch Med, Dept Mol Biol & Genet, Baltimore, MD 21287 USA Johns Hopkins Univ Baltimore MD USA 21287 Genet, Baltimore, MD 21287 USA Univ Michigan, Sch Med, Dept Cell & Dev Biol, Ann Arbor, MI USA Univ Michigan Ann Arbor MI USA , Dept Cell & Dev Biol, Ann Arbor, MI USA Univ Michigan, Coll Literature, Dept Biol, Ann Arbor, MI 48109 USA Univ Michigan Ann Arbor MI USA 48109 , Dept Biol, Ann Arbor, MI 48109 USA
Titolo Testata:
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
fascicolo: 2, volume: 42, anno: 2001,
pagine: 481 - 487
SICI:
0146-0404(200102)42:2<481:IACOAZ>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
PHOTORECEPTOR-SPECIFIC EXPRESSION; LEBER CONGENITAL AMAUROSIS; DANIO-RERIO; EMBRYONIC ZEBRAFISH; ADULT ZEBRAFISH; EYE DEVELOPMENT; CRX; MUTATIONS; RETINA; TRANSCRIPTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Zack, DJ Johns Hopkins Univ, Sch Med, Dept Ophthalmol, 600 N Wolfe St,Maumenee 809,Baltimore, MD 21287 USA Johns Hopkins Univ 600 N Wolfe St,Maumenee809 Baltimore MD USA 21287
Citazione:
Y.H. Liu et al., "Isolation and characterization of a zebrafish homologue of the cone rod homeobox gene", INV OPHTH V, 42(2), 2001, pp. 481-487

Abstract

PURPOSE. To isolate and characterize a zebrafish Crx homologue. Mammalian Crx genes are expressed specifically in photoreceptors and pinealocytes, regulate photoreceptor gene expression, are necessary for normal photoreceptor differentiation, and when mutated cause a variety of photoreceptor degenerations. METHODS. A zebrafish retinal cDNA library was screened with a human CRX cDNA probe. Radiation hybrid mapping, Northern blot analysis, in situ hybridization and transient transfection studies were performed using standard methods. RESULTS. Based on amino acid sequence comparisons, zebrafish crx shows 50%identity with human CRX, and 85% identity in the homeodomain. A phylogenetic analysis indicates that zebrafish crx is most closely related to the mammalian Crx proteins, and more distantly related to the Otx proteins. Zebrafish crx maps between 49.6 and 54.5 cm from the top of linkage group LG05C, a map position consistent with the location of the mouse and human CRX genes. Northern blot analysis and in situ hybridization indicate that zebrafishcrx is expressed in the retina. and pineal gland. In adult zebrafish, crx expressed by both rods and cones in the outer nuclear layer, and in cells in the outer zone of the inner nuclear layer, in the region occupied by bipolar cells. Similar to mammalian Crx, zebrafish crx interacts with neural retinal leucine zipper (Nrl) to activate, although weakly, rhodopsin promoteractivity. CONCLUSIONS. Based on molecular phylogeny, chromosomal location, expression pattern, and ability to activate rhodopsin promoter activity in transienttransfection assays, zebrafish crx appears to be an orthologue and functional homologue of mammalian Crx.

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Documento generato il 31/03/20 alle ore 04:18:39