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Titolo:
Detection of endogenous 12-hydroxyeicosatrienoic acid in human tear film
Autore:
Mieyal, PA; Dunn, MW; Schwartzman, ML;
Indirizzi:
New York Med Coll, Dept Pharmacol, Valhalla, NY 10595 USA New York Med Coll Valhalla NY USA 10595 Pharmacol, Valhalla, NY 10595 USA
Titolo Testata:
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
fascicolo: 2, volume: 42, anno: 2001,
pagine: 328 - 332
SICI:
0146-0404(200102)42:2<328:DOE1AI>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
RABBIT CORNEAL EPITHELIUM; CONTACT-LENS MODEL; ARACHIDONIC-ACID; 12(S)-HYDROXY-5,8,10,14-EICOSATETRAENOIC ACID; 12(S)-HYDROXYEICOSATETRAENOIC ACID; 12(R)-HYDROXYEICOSATRIENOIC ACID; POLYMORPHONUCLEAR LEUKOCYTES; INFLAMMATORY EICOSANOIDS; METABOLITES; INHIBITION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Schwartzman, ML New York Med Coll, Dept Pharmacol, Valhalla, NY 10595 USA New York Med Coll Valhalla NY USA 10595 lla, NY 10595 USA
Citazione:
P.A. Mieyal et al., "Detection of endogenous 12-hydroxyeicosatrienoic acid in human tear film", INV OPHTH V, 42(2), 2001, pp. 328-332

Abstract

PURPOSE. Increased production of 12-hydroxyeicosatetraenoic acid [12(R)-HETE] and 12-hydroxyeicosatrienoic acid [12(R)-HETrE] positively correlates with the in vivo progression of ocular surface inflammation in rabbits. Tearfilm was collected from human subjects with inflamed eyes to determine whether these eicosanoids could be detected from endogenous sources. METHODS. Control and inflamed eyes were assessed and assigned a subjectiveinflammatory score. Tears were collected and extracted with an internal standard. Single-ion-monitoring gas chromatography-mass spectrometry (SIM-GC-MS) was performed to quantitate endogenous levels of 12-HETE and 12-HETrE. RESULTS. 12-HETrE was detected in the tear film of both control and inflamed eyes, with the mean level being seven times higher in inflamed tears. 12-HETE was not detected in control tears and was detected in only 6 of 38 inflamed-eye tear samples. CONCLUSIONS. The current findings demonstrate that the human eye produces detectable amounts of 12-HETrE, which is released into the tear flow. The increased levels of 12-HETrE associated with ocular surface inflammation suggest that this eicosanoid may contribute to inflammation of the ocular surface in humans.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 18:14:17