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Titolo:
Reduction in intestinal leukocyte adherence in rat experimental endotoxemia by treatment with the 21-aminosteroid U-74389G
Autore:
Lehmann, C; Georgiew, A; Weber, M; Birnbaum, J; Kox, WJ;
Indirizzi:
Univ Klinikum Berlin, Charite, Klin Anasthesiol & Operat Intens Med, D-10098 Berlin, Germany Univ Klinikum Berlin Berlin Germany D-10098 Med, D-10098 Berlin, Germany
Titolo Testata:
INTENSIVE CARE MEDICINE
fascicolo: 1, volume: 27, anno: 2001,
pagine: 258 - 263
SICI:
0342-4642(200101)27:1<258:RIILAI>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-NECROSIS-FACTOR; ENDOTHELIAL CELL-INTERACTIONS; MULTIPLE-ORGAN-FAILURE; OXYGEN RADICALS; ADHESION; MECHANISMS; MODULATION; TIRILAZAD; MUSCLE; SEPSIS;
Keywords:
endotoxemia; oxygen free radicals; antioxidants; U-74389G; intravital fluorescence microscopy; leukocyte adhesion;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Lehmann, C Univ Klinikum Berlin, Charite, Klin Anasthesiol & Operat IntensMed, Campus Charite Mitte, D-10098 Berlin, Germany Univ Klinikum Berlin Campus Charite Mitte Berlin Germany D-10098
Citazione:
C. Lehmann et al., "Reduction in intestinal leukocyte adherence in rat experimental endotoxemia by treatment with the 21-aminosteroid U-74389G", INTEN CAR M, 27(1), 2001, pp. 258-263

Abstract

Objectives:To investigate leukocyte adherence in intestinal venules in experimental endotoxemia after treatment with the 21-aminosteroid U-74389G. Design and setting: Prospective, randomized, controlled animal study in anexperimental laboratory. Subjects: Twenty-one male Wistar rats weighing 190 +/- 40 g. Interventions: The rats were divided equally into three groups: (a) control group, (b) endotoxemia (5 mg/ kg lipopolysacharide from Escherichia coli O55:B5), and (c) endotoxemia and U-74389G administration 30 min before (3 mg/kg) and 60 min after endotoxin challenge (1.5 mg/ kg). Measurements and main results: The distal small intestine of the animals was examined using intravital fluorescence videomicroscopy 2 h after endotoxin challenge. Leukocytes were stained in vivo by means of rhodamine 6G. In the endotoxemic animals we observed a fourfold increase in the count of firmly adherent leukocytes in submucosal postcapillary and collecting venules. Treatment with the 21-aminosteroid U-74389G significantly attenuated the count of sticking leukocytes in the collecting venules (control, 61 +/- 10 cells/mm(2); lipopolysaccharide, 237 +/- 42 cells/mm(2); U-74389G 125 +/- 9 cells/mm(2);p < 0.05). In these venules leukocyte rolling behavior was comparable to that in the control group without endotoxin challenge. Conclusions: Administration of U-743890, which has radical scavenging properties, attenuates leukocyte adherence in selected populations of intestinal venules which is found increased during endotoxemia. Thus, 21-aminosteroids may have an impact in the treatment of endotoxin-induced intestinal injury.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 12:05:55