Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Maintenance of GABA receptor function of small-diameter cockroach neurons by adenine nucleotides
Autore:
Watson, GB; Salgado, VL;
Indirizzi:
Dow AgroSci Discovery Res, Indianapolis, IN 46268 USA Dow AgroSci Discovery Res Indianapolis IN USA 46268 napolis, IN 46268 USA
Titolo Testata:
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
fascicolo: 2, volume: 31, anno: 2001,
pagine: 207 - 212
SICI:
0965-1748(200102)31:2<207:MOGRFO>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
CENTRAL-NERVOUS-SYSTEM; PROTEIN-PHOSPHORYLATION; DROSOPHILA; PHARMACOLOGY; SUBTYPE;
Keywords:
gamma-aminobutyric acid (GABA); cockroach; neuronal; phosphorylation; adenosine 5 '-triphosphate (ATP); adenine nucleotides;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Watson, GB Dow AgroSci Discovery Res, 9330 Zionsville Rd, Indianapolis, IN46268 USA Dow AgroSci Discovery Res 9330 Zionsville Rd Indianapolis IN USA46268
Citazione:
G.B. Watson e V.L. Salgado, "Maintenance of GABA receptor function of small-diameter cockroach neurons by adenine nucleotides", INSEC BIO M, 31(2), 2001, pp. 207-212

Abstract

Small diameter (<20 <mu>m) neurons from the sixth abdominal ganglion of the American cockroach, Periplaneta americana, were enzymatically isolated and responses to exogenously applied gamma -aminobutyric acid (GABA) were recorded using the whole-cell patch clamp technique. With a minimal intracellular medium, responses to repeated applications ofGABA decreased to zero within a few minutes. The rate of rundown of GABA responses was decreased by the intracellular inclusion of the phosphatase inhibitors microcystin and okadaic acid, suggesting that phosphorylation is necessary for the maintenance of cockroach GABA receptor function. ATP (5 mM) prevented GABA response rundown. ADP (5 mM) also slowed GABA response rundown, but responses stabilized at a level about half that seen with ATP. In the presence of protein kinase A inhibitory peptide (PKI), ATP was onlyas efficacious as ADP in slowing rundown. PKI, had no effect on the ability of ADP to slow rundown, suggesting that the beta -phosphate of ADP is notinvolved in PKA-dependent phosphorylation of the GABA receptor. These results suggest that in cockroach neurons, GABA receptor function ismaintained intracellularly by adenine nucleotides, not only by phosphorylation, but also possibly by an interaction with a nucleotide recognition site unrelated to PKA-dependent phosphorylation. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 16:47:10