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Titolo:
A current perspective on insect gene transformation
Autore:
Handler, AM;
Indirizzi:
USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA USDA ARS Gainesville FL USA 32608 Vet Entomol, Gainesville, FL 32608 USA
Titolo Testata:
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
fascicolo: 2, volume: 31, anno: 2001,
pagine: 111 - 128
SICI:
0965-1748(200102)31:2<111:ACPOIG>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSPOSABLE ELEMENT MARINER; NON-DROSOPHILID INSECTS; SITE-SPECIFIC RECOMBINATION; GREEN-FLUORESCENT PROTEIN; PSEUDOTYPED RETROVIRAL VECTORS; GERM-LINE TRANSFORMATION; MEDITERRANEAN FRUIT-FLY; DNA-BINDING DOMAIN; ANOPHELES-GAMBIAE; HERMES ELEMENT;
Keywords:
insect gene transfer; germ-line transformation; transposable elements; paratransgenesis; viral vectors; fluorescent proteins;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
154
Recensione:
Indirizzi per estratti:
Indirizzo: Handler, AM USDA ARS, Ctr Med Agr & Vet Entomol, 1700 SW 23rd Dr, Gainesville, FL 32608 USA USDA ARS 1700 SW 23rd Dr Gainesville FL USA 32608 FL 32608 USA
Citazione:
A.M. Handler, "A current perspective on insect gene transformation", INSEC BIO M, 31(2), 2001, pp. 111-128

Abstract

The genetic transformation of non-drosophilid insects is now possible withseveral systems, with germ-line transformation reported in published and unpublished accounts for about 12 species using four different transposon vectors. For some of these species, transformation can now be considered routine. Other vector systems include viruses and bacterial symbionts that havedemonstrated utility in species and applications requiring transient expression, and for some, the potential exists for genomic integration. Many of these findings are quite recent, presenting a dramatic turning point in ourability to study and manipulate agriculturally and medically important insects. This review discusses these findings from the perspective of all the contributions that has made this technology a reality, the research that has yet to be done for its safe and efficient use in a broader range of species, and an overview of the available methodology to effectively utilize these systems. Published by Elsevier Science Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 12:58:28