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Titolo:
Heart-specific splice-variant of a human mitochondrial ribosomal protein (mRNA processing; tissue specific splicing)
Autore:
Spirina, O; Bykhovskaya, Y; Kajava, AV; OBrien, TW; Nierlich, DP; Mougey, EB; Sylvester, JE; Graack, HR; Wittmann-Liebold, B; Fischel-Ghodsian, N;
Indirizzi:
Cedars Sinai Med Ctr, Burns & Allen Res Inst, Med Genet Birth Defects Ctr,Ahmanson Dept Pediat, Steven Spielberg Pediat Res Ctr, Los Angeles, CA 90048 USA Cedars Sinai Med Ctr Los Angeles CA USA 90048 , Los Angeles, CA 90048 USA Univ Calif Los Angeles, Sch Med, Los Angeles, CA USA Univ Calif Los Angeles Los Angeles CA USA , Sch Med, Los Angeles, CA USA NIH, CIT, Ctr Mol Modeling, Bethesda, MD USA NIH Bethesda MD USANIH, CIT, Ctr Mol Modeling, Bethesda, MD USA Univ Florida, Gainesville, FL USA Univ Florida Gainesville FL USAUniv Florida, Gainesville, FL USA Univ Calif Los Angeles, Los Angeles, CA USA Univ Calif Los Angeles Los Angeles CA USA s Angeles, Los Angeles, CA USA Nemours Childrens Clin, Jacksonville, FL USA Nemours Childrens Clin Jacksonville FL USA ns Clin, Jacksonville, FL USA Max Delbruck Ctr Mol Med, Berlin, Germany Max Delbruck Ctr Mol Med Berlin Germany ck Ctr Mol Med, Berlin, Germany
Titolo Testata:
GENE
fascicolo: 2, volume: 261, anno: 2000,
pagine: 229 - 234
SICI:
0378-1119(200012)261:2<229:HSOAHM>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
DATABASE; RNA;
Keywords:
heart-specific splice-variant; human mitochondrial ribosomal protein; mRNA processing; tissue specific splicing;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Fischel-Ghodsian, N Cedars Sinai Med Ctr, Dept Pediat, WT 1165,8700 Beverly Blvd, Los Angeles,CA 90048 USA Cedars Sinai Med Ctr WT 1165,8700 Beverly Blvd Los Angeles CA USA 90048
Citazione:
O. Spirina et al., "Heart-specific splice-variant of a human mitochondrial ribosomal protein (mRNA processing; tissue specific splicing)", GENE, 261(2), 2000, pp. 229-234

Abstract

It has been proposed that splice-variants of proteins involved in mitochondrial RNA processing and translation may be involved in the tissue specificity of mitochondrial DNA disease mutations (Fischel-Ghodsian, 1998. Mel. Genet. Metab. 65, 97-104). To identify and characterize the structural components of mitochondrial RNA processing and translation, the Mammalian Mitochondrial Ribosomal Consortium has been formed. The 338 amino acid (aa) residues long MRP-LS was identified (O'Brien et al., 1999. J. Biol. Chem. 274, 36043-36051), and its transcript was screened for tissue specific splice-variants. Screening of the EST databases revealed a single putative splice-variant, due to the insertion of an exon consisting of 89 nucleotides prior to the last exon. Screening of multiple cDNA libraries revealed this inserted exon to be present only in heart tissue, in addition to the predominant MRP-LS transcript. Sequencing of this region confirmed the EST sequence, and showed in the splice-variant a termination triplet at the beginning of the last exon. Thus the inserted exon replaces the coding sequence of the regular last exon, and creates a new 353 aa long protein (MRP-L5V1). Sequence analysis and 3D modeling reveal similarity between MRP-LS and threonyl-t-RNA synthetases, and a likely RNA binding site within MRP-LS, with the C-terminus in proximity to the RNA binding site, Sequence analysis of MRP-L5V1 also suggests a likely transmembrane domain at the C-terminus. Thus it is possible that the MRP-L5V1 C-terminus could interfere with RNA binding and may have gained a transmembrane domain. Further studies will be required to elucidate the functional significance of MRP-L5V1. (C) 2000 Elsevier Science B.V. All rights reserved.

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Documento generato il 02/12/20 alle ore 15:31:30