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Titolo:
Differences between cysteine and homocysteine in the induction of deoxyribose degradation and DNA damage
Autore:
Muniz, P; Saez, P; Iradi, A; Vina, J; Oliva, MR; Saez, GT;
Indirizzi:
Univ Valencia, Fac Med, Dept Biochem & Mol Biol, E-46010 Valencia, Spain Univ Valencia Valencia Spain E-46010 & Mol Biol, E-46010 Valencia, Spain Univ Valencia, Fac Med, Dept Physiol, E-46010 Valencia, Spain Univ Valencia Valencia Spain E-46010 pt Physiol, E-46010 Valencia, Spain
Titolo Testata:
FREE RADICAL BIOLOGY AND MEDICINE
fascicolo: 4, volume: 30, anno: 2001,
pagine: 354 - 362
SICI:
0891-5849(20010215)30:4<354:DBCAHI>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
N-ACETYL CYSTEINE; STRAND BREAKS; CYTO-TOXICITY; COPPER; GLUTATHIONE; 8-HYDROXY-2'-DEOXYGUANOSINE; GENERATION; OXIDATION; IONS; SYSTEM;
Keywords:
cysteine; homocysteine; autoxidation; 8-hydroxy-2 '-deoxyguanosine; oxygen free radicals; DNA damage; deoxyribose;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Saez, GT Univ Valencia, Fac Med, Dept Biochem & Mol Biol, Avda Blasco Ibanez 17, E-46010 Valencia, Spain Univ Valencia Avda Blasco Ibanez 17 Valencia Spain E-46010 Spain
Citazione:
P. Muniz et al., "Differences between cysteine and homocysteine in the induction of deoxyribose degradation and DNA damage", FREE RAD B, 30(4), 2001, pp. 354-362

Abstract

The effect of two naturally occurring thiols, such as cysteine and homocysteine, has been examined for their ability to induce deoxyribose degradation and DNA. damage. Copper(II) ions have been added to incubation mixtures and oxygen consumption measurements have been performed in order to correlate the observed damaging effects with the rate of metal catalyzed thiol oxidation. Ascorbic acid plus copper has been used as a positive control of deoxyribose and DNA oxidation due to reactive oxygen species. Cysteine or homocysteine in the presence of copper ions induce the degradation of deoxyribose and the yield of 8-hydroxy-2'-deoxyguanosine (8-OHdG), although important differences are observed between the two thiols tested, homocysteine being less reactive than cysteine. DNA cleavage is induced by cysteine in the presence of copper(II) ions but not by homocysteine. Catalase and thiourea, but not superoxide dismutase (SOD), were shown to inhibit the damaging effects of cysteine on deoxyribose or DNA suggesting that H2O2 and (OH)-O-. radicals are responsible for the observed induced damage. The results indicatethat there are differences between the damaging effects of the two thiols tested towards deoxyribose and DNA damage. The pathophysiological importance will be discussed. (C) 2001 Elsevier Science Inc.

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Documento generato il 26/11/20 alle ore 11:43:35