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Titolo:
Peritoneal B-cell development depends on strain, radiation, and time
Autore:
Stickler, M; Harding, F;
Indirizzi:
Genencor Int Inc, Dept Immunol, Palo Alto, CA 94304 USA Genencor Int Inc Palo Alto CA USA 94304 Immunol, Palo Alto, CA 94304 USA
Titolo Testata:
EXPERIMENTAL HEMATOLOGY
fascicolo: 2, volume: 29, anno: 2001,
pagine: 221 - 227
SICI:
0301-472X(200102)29:2<221:PBDDOS>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
TOTAL-BODY IRRADIATION; VERSUS-HOST DISEASE; ADULT BONE-MARROW; LY-1 B; LINEAGE; MICE; PRECURSORS; SEQUENCES; DISTINCT; ORIGIN;
Keywords:
B-1a cells; engraftment; adult bone marrow; SCID; RAG-2;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Stickler, M Genencor Int Inc, Dept Immunol, 925 Page Mill Rd, Palo Alto, CA 94304 USA Genencor Int Inc 925 Page Mill Rd Palo Alto CA USA 94304 4 USA
Citazione:
M. Stickler e F. Harding, "Peritoneal B-cell development depends on strain, radiation, and time", EXP HEMATOL, 29(2), 2001, pp. 221-227

Abstract

Objective. B-1a, B-1b, and B-2 cells represent the three B-cell subsets inmice. Previous studies have demonstrated that peritoneal B-la cell development is absent, or nearly so, from adult bone marrow transfers into irradiated adult hosts. The majority of these studies have been performed under a limited set of conditions with irradiated host mice. Here we examined that under a variety of conditions, peritoneal R-la cells can develop in significant numbers From adult bone marrow transfers into severe combined immunodeficient (SCID) and recombination activation gene 2(-) (RAG-2(-))mice, Materials and Methods. Adult hone marrow was transferred into various strains ofirradiated and nonirradiated adult immunodeficient RAG-2(-) and SCID mice. Peritoneal B-cell engraftment was examined by fluorescein-activated cell sorting analysis and unpaired t-tests were used to determine significant differences of B-cell engraftment among the various conditions of cell transfer. Results. The level of B-1a cell engraftment was variously affected by the type of host immunodeficiency, the combination of donor and host strains,and the time allowed for engraftment, Irradiation of SCID, but not RAG-2-,host mice inhibited B-1a-cell engraftment, Additionally, decreasing the number of bone marrow progenitor cells transferred was not found to preferentially affect B-1a cell development in irradiated RAG-2(-) hosts, Conclusion. In the context of these strains, we conclude that adult murine bone marrow contains progenitors that have the capacity to reconstitute peritoneal B-1a cell populations to donor levels, (C) 2001 international Society for Experimental Hematology. Published by Elsevier Science inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 10:59:18