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Titolo:
Acid stimulates E-cadherin surface expression on gastric epithelial cells to stabilize barrier functions via influx of calcium
Autore:
Mizuno, M; Okayama, N; Kasugai, K; Yoshida, T; Miyata, M; Nitta, M; Kataoka, H; Akita, S; Joh, T; Itoh, M; Asai, K; Kato, T;
Indirizzi:
Aichi Med Univ, Sch Med, Dept Internal Med 2, Nagakute, Aichi 4801195, Japan Aichi Med Univ Nagakute Aichi Japan 4801195 agakute, Aichi 4801195, Japan
Titolo Testata:
EUROPEAN JOURNAL OF GASTROENTEROLOGY & HEPATOLOGY
fascicolo: 2, volume: 13, anno: 2001,
pagine: 127 - 136
SICI:
0954-691X(200102)13:2<127:ASESEO>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTRACELLULAR PH; BARRETTS-ESOPHAGUS; ENDOTHELIAL-CELLS; GENE-EXPRESSION; DOWN-REGULATION; RAT; ADHESION; DEDIFFERENTIATION; ADENOCARCINOMA; ACIDIFICATION;
Keywords:
cell culture insert; extracellular calcium; intracellular calcium; RT-PCR; western blotting analysis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Mizuno, M Aichi Med Univ, Sch Med, Dept Internal Med 2, 21 Karimata, Nagakute, Aichi4801195, Japan Aichi Med Univ 21 Karimata Nagakute Aichi Japan 4801195 5, Japan
Citazione:
M. Mizuno et al., "Acid stimulates E-cadherin surface expression on gastric epithelial cells to stabilize barrier functions via influx of calcium", EUR J GASTR, 13(2), 2001, pp. 127-136

Abstract

Background and aims E-cadherin, which is a [Ca2+]-dependent, homotypic cell-cell adhesion molecule, is expressed in gastrointestinal epithelial cells. Much has been learned about the down-regulation of E-cadherin expression in gastrointestinal tumours, Barrett's oesophageal dysplasia, and Crohn's disease, but the functions of this molecule in normal gastrointestinal mucosa are less known. Methods In this study, we investigated the relationship between E-cadherinexpression and permeability using rat cultured gastric and intestinal epithelial cells following a 30-min exposure to various pH solutions. We also investigated the participation of [Ca2+] in these events. Results E-cadherin expression increased under acid (pH 4) but not alkali (pH 10 or 11) exposure only for gastric epithelial cells. Gastric epithelialpermeability was maintained only against acid exposure while intestinal permeability increased under both conditions. Transient influx of [Ca2+] was only observed for gastric epithelial cells just after acid exposure. Conclusions These findings suggest that E-cadherin expression on gastric epithelium stabilizes the epithelial barrier against acid, probably through influx of [Ca2+]. This event is thought to be one of the protective mechanisms in gastric mucosa against acid back-diffusion, which is one of the causes of peptic ulcer formation. Eur J Gastroenterol Hepatol 13:127-136 (C) 2001 Lippincott Williams & Wilkins.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/04/20 alle ore 11:26:26