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Titolo:
Receptor-like protein tyrosine phosphatase gamma (RPTP gamma). but not PTPzeta/PTP beta, inhibits nerve-growth-factor-induced neurite outgrowth in PC12D cells
Autore:
Shintani, T; Maeda, N; Noda, M;
Indirizzi:
Natl Inst Basic Biol, Div Mol Neurobiol, Okazaki, Aichi 4448585, Japan Natl Inst Basic Biol Okazaki Aichi Japan 4448585 ki, Aichi 4448585, Japan Grad Univ Adv Studies, Dept Mol Biomech, Okazaki, Aichi, Japan Grad Univ Adv Studies Okazaki Aichi Japan Biomech, Okazaki, Aichi, Japan
Titolo Testata:
DEVELOPMENTAL NEUROSCIENCE
fascicolo: 1, volume: 23, anno: 2001,
pagine: 55 - 69
SICI:
0378-5866(200101/02)23:1<55:RPTPG(>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOLECULE HB-GAM; CHONDROITIN SULFATE PROTEOGLYCAN; HIGH-AFFINITY BINDING; DEVELOPING RAT-BRAIN; ZETA/RPTP-BETA; RPTP-BETA; 6B4 PROTEOGLYCAN/PHOSPHACAN; PHEOCHROMOCYTOMA CELLS; SIGNAL-TRANSDUCTION; KINASE;
Keywords:
receptor-like protein tyrosine phosphatase gamma protein tyrosine phosphatase zeta; PC12D cells; neurite extension; nerve growth factor; p13(suc1); protein kinase C;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
59
Recensione:
Indirizzi per estratti:
Indirizzo: Noda, M Natl Inst Basic Biol, Div Mol Neurobiol, 38 Nishigonaka, Okazaki, Aichi 4448585, Japan Natl Inst Basic Biol 38 Nishigonaka Okazaki Aichi Japan 4448585 an
Citazione:
T. Shintani et al., "Receptor-like protein tyrosine phosphatase gamma (RPTP gamma). but not PTPzeta/PTP beta, inhibits nerve-growth-factor-induced neurite outgrowth in PC12D cells", DEV NEUROSC, 23(1), 2001, pp. 55-69

Abstract

Receptor-like protein tyrosine phosphatase gamma (RPTP gamma) and PTP zeta/RPTP beta are RPTPs which structurally resemble each other and form a distinct RPTP family. Both molecules are highly expressed in the central nervous system (CNS), though RPTP gamma is distributed also in several peripheral tissues. To date, the functional differences between RPTP gamma and PTP zeta in neuronal cells have not been made clear because their substrate and ligand molecules have not been fully elucidated. To address this issue, we established PC12D cell transfectants stably expressing rat RPTP gamma or PTP zeta and analyzed the effects on cellular response to nerve growth factor(NGF). Compared with the parent PC12D cells which extend neurites vigorously in response to NGF, the transfectants expressing RPTP gamma showed a most no neurite outgrowth. In contrast, neurite extension in PTP zeta -expressing clones on NGF treatment was the same as in parent cells. We investigated differences in tyrosine phosphorylation levels in the cellular proteins in these cells after the NGF treatment before morphological charges appeared. Despite the lack of a response, major proteins and MAP kinase in RPTP gamma -expressing PC12D cells displayed normal tyrosine phosphorylation changes on NGF treatment. However, tyrosine phosphorylation levels in the proteincomponents purified with p13(suc1) agarose (p13(suc)1 complex) from RPTP gamma -expressing cells were different from those of the control cells. (1) Tyrosine-phosphorylation levels of 140- and 117-kD proteins were significantly reduced. (2) Rapid tyrosine phosphorylation of 58-kD protein induced byNGF was absent. (3) Activities of tyrosine kinases and protein kinase C inthe p13(suc)1 complex were markedly reduced. We found that the p13(suc1) complex also contained cytoskeletal proteins such as MAP2 and neurofilaments, but their phosphorylation levels were not different. These results indicate that RPTP gamma and PTP zeta have different substrate specificities, andRPTP gamma inhibits NGF-induced neurite outgrowth of PC12D cells through modulation of the p13(suc1) complex. Copyright (C) 2001 S. Karger AG, Basel.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 17:34:04