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Titolo:
The orphan nuclear receptor NOR-1 interacts with the homeobox containing protein Six3
Autore:
Ohkura, N; Ohkubo, T; Maruyama, K; Tsukada, T; Yamaguchi, K;
Indirizzi:
Natl Canc Ctr, Res Inst, Div Growth Factor, Chuo Ku, Tokyo 1040045, Japan Natl Canc Ctr Tokyo Japan 1040045 Factor, Chuo Ku, Tokyo 1040045, Japan
Titolo Testata:
DEVELOPMENTAL NEUROSCIENCE
fascicolo: 1, volume: 23, anno: 2001,
pagine: 17 - 24
SICI:
0378-5866(200101/02)23:1<17:TONRNI>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
NERVE GROWTH-FACTOR; NGFI-B; TRANSCRIPTION FACTOR; DEVELOPING BRAIN; GENE; EXPRESSION; SUPERFAMILY; HOMEODOMAIN; MEMBER; NUR77;
Keywords:
NOR-1; Six3; orphan nuclear receptor; homeobox gene; NGFI-B family;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Ohkura, N Natl Canc Ctr, Res Inst, Div Growth Factor, Chuo Ku, 5-1-1 Tsukiji, Tokyo 1040045, Japan Natl Canc Ctr 5-1-1 Tsukiji Tokyo Japan 1040045 1040045, Japan
Citazione:
N. Ohkura et al., "The orphan nuclear receptor NOR-1 interacts with the homeobox containing protein Six3", DEV NEUROSC, 23(1), 2001, pp. 17-24

Abstract

Neuron-derived orphan receptor 1 (NOR-1) is a member of the NGFI-B subfamily within the nuclear receptor superfamily. In order to identify cofactors that associate with NOR-1 in the fetal forebrain, we tested a yeast two-hybrid system with the NOR-1 cDNA fragment lacking a transactivating domain asa bait. By screening of the rat fetal brain embryonic day 17 library, a rat homologue of Six3 was identified as an associated protein. We demonstrated that NOR-1 interacted with Six3 in yeast and in vitro, and the association was required for the DNA binding and AF2 domains of NOR-1. Regarding the other members of the family (NGFI-B and RNR-1), association with Six3 was not observed in yeast. In addition, cotransfection experiments with Six3 andNOR-1 indicated that Six3 had a negative activity against the transactivation by NOR-1 through the NBRE response element in a dose-dependent manner. The overlap in expression of NOR-1 and Six3 was mainly detected in the rat fetal forebrain on embryonic day 18. Thereafter, the expression of both genes diminished rapidly. These results suggest that a dimer consisting of a homeobox containing protein Six3 and transcriptional factor NOR-1 might regulate gene expression during the late stage of the fetal forebrain development. This study provides, after the association of Ftz and Ftz-F1 in Drosophila, another example of a dimer formation of a homeobox protein and an orphan nuclear receptor. Copyright (C) 2001 S. Karger AG, Basel.

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Documento generato il 02/10/20 alle ore 02:01:18