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Titolo:
Toxinological studies of the venom from Cassiopea xamachana nematocysts isolated by flow cytometry
Autore:
Radwan, FFY; Burnett, JW;
Indirizzi:
Univ Maryland, Sch Med, Dept Dermatol, Baltimore, MD 21201 USA Univ Maryland Baltimore MD USA 21201 pt Dermatol, Baltimore, MD 21201 USA
Titolo Testata:
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
fascicolo: 1, volume: 128, anno: 2001,
pagine: 65 - 73
SICI:
1532-0456(200101)128:1<65:TSOTVF>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEINS; ALGAE;
Keywords:
jellyfish; Cassiopea; flow cytometry; venoms; toxins; Red Sea; nematocyst; hemolysin; capillary electrophoresis; cytosensor;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
17
Recensione:
Indirizzi per estratti:
Indirizzo: Burnett, JW Univ Maryland, Sch Med, Dept Dermatol, 405 W Redwood St,6th Floor, Baltimore, MD 21201 USA Univ Maryland 405 W Redwood St,6th Floor Baltimore MD USA 21201
Citazione:
F.F.Y. Radwan e J.W. Burnett, "Toxinological studies of the venom from Cassiopea xamachana nematocysts isolated by flow cytometry", COMP BIOC C, 128(1), 2001, pp. 65-73

Abstract

The tentacle epithelial tissue of Cassiopea xamachana contains nematocystsand symbiotic algal particles. These two structures were dissociated, analyzed and sorted by flow cytometry. A simple separating method was developedutilizing the algal chlorophyll autofluorescence and the nematocysts' fluorescence after the uptake of fluorescent stains. A five-fold increase in mouse lethality; significantly more potent hemolytic and cytosensing activities; as well as a cleanup in the capillary electropherogram and SDS gel profiles for the crude nematocyst venom preparations prepared by fluorescence activated cell sorting (FACS), was observed relative to alternative methods. Because the hemolytic potency of pre-sorting nematocyst venom was minimal and the post-sorting counterpart was significantly positive, the possibility that algae inhibited the venom's toxinological activity was considered. (C) 2001 Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 13:42:05