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Titolo:
Replicative response, immunophenotype, and functional activity of monocyte-derived versus CD34(+)-derived dendritic cells following exposure to various expansion and maturational stimuli
Autore:
Chen, B; Stiff, P; Sloan, G; Kash, J; Manjunath, R; Pathasarathy, M; Oldenburg, D; Foreman, KE; Nickoloff, BJ;
Indirizzi:
Loyola Univ, Med Ctr, Cardinal Bernardin Canc Ctr, Skin Canc Res Program,Dept Pathol, Maywood, IL 60153 USA Loyola Univ Maywood IL USA 60153 ogram,Dept Pathol, Maywood, IL 60153 USA Loyola Univ, Med Ctr, Dept Med, Div Hematol Oncol, Maywood, IL 60153 USA Loyola Univ Maywood IL USA 60153 Div Hematol Oncol, Maywood, IL 60153 USA
Titolo Testata:
CLINICAL IMMUNOLOGY
fascicolo: 2, volume: 98, anno: 2001,
pagine: 280 - 292
SICI:
1521-6616(200102)98:2<280:RRIAFA>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEMATOPOIETIC PROGENITOR CELLS; PERIPHERAL-BLOOD; BONE-MARROW; LANGERHANS CELLS; PROTEIN ANTIGENS; APOPTOTIC CELLS; CD34(+) CELLS; EX-VIVO; GENERATION; CULTURES;
Keywords:
dendritic cells; immunotherapy; vaccines; CD34; CD40L; monocytes;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Nickoloff, BJ Loyola Univ, Med Ctr, Cardinal Bernardin Canc Ctr, Skin CancRes Program,Dept Pathol, 2160 S 1st Ave, Maywood, IL 60153 USA Loyola Univ2160 S 1st Ave Maywood IL USA 60153 IL 60153 USA
Citazione:
B. Chen et al., "Replicative response, immunophenotype, and functional activity of monocyte-derived versus CD34(+)-derived dendritic cells following exposure to various expansion and maturational stimuli", CLIN IMMUNO, 98(2), 2001, pp. 280-292

Abstract

Dendritic cells (DCs), generated ex vivo from blood mononuclear cells (PBMC) or CD34(+) stem cells, are being used to develop novel immunotherapies. To establish optimal DC generation, a direct comparison of the optimal cellsource, culture conditions, and maturation stimuli was performed, utilizing phenotypic and functional assays as end points. Plastic adherent monocytes from PBMC were expanded in a serum-free medium (X-Vivo 10) for 7 days using GM-CSF/IL-4; CD34+ cells were expanded for 14 days using GM-CSF/IL-4/Flt3L, in either X-Vivo 10 alone or with albumin or autologous plasma. Expanded DC from both cell sources were matured for 7 days with CD40L or IFN-alpha/TNF-alpha. Starting from 2 x 10(7) monocytes, the optimal expansion/maturation process yielded 1.73 +/- 0.52 x 10(6) CD86(+) DC. Optimal expansion of CD34(+) cells (83.9 +/- 25.0-fold) was achieved using X-Vivo 10 with 5% plasma, matured with CD40L, and yielded 10.68 +/- 2.72 x 10(6) CD86(+) DC from 1 x 10(6) CD34(+) cells. Mature DC from PBMC or CD34(+) cells had similar enhanced expression of MHC class II HLA-DR, CD80, CD83, and CD86 and werepotent stimulators of mixed lymphocyte reactions. Prior to maturation, allgroups of DC actively phagocytosed apoptotic melanoma cells (approximately50% of HLA-DR+). CD34(+) DC matured with CD40L or IFN-alpha /TNF-alpha hadreduced phagocytic capability (34 and 31% of HLA-DR+ DC, respectively). Similar expansion and functional activity was found using cryopreserved DC precursors, cultured in gas permeable bags. We conclude that both cell lineages produce potent mature DC, permitting exploration of the optimal clinicalstrategy to trigger anti-tumor immune responses in patients with malignancies. (C) 2000 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 10:08:04