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Titolo:
The phosphodiesterase inhibitors pentoxifylline and rolipram suppress macrophage activation and nitric oxide production in vitro and in vivo
Autore:
Beshay, E; Croze, F; Prudhomme, GJ;
Indirizzi:
McGill Univ, Dept Pathol, Montreal, PQ H3A 2B4, Canada McGill Univ Montreal PQ Canada H3A 2B4 thol, Montreal, PQ H3A 2B4, Canada
Titolo Testata:
CLINICAL IMMUNOLOGY
fascicolo: 2, volume: 98, anno: 2001,
pagine: 272 - 279
SICI:
1521-6616(200102)98:2<272:TPIPAR>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-NECROSIS-FACTOR; MRL-LPR/LPR MICE; INSULIN-SECRETION; AUTOIMMUNE ENCEPHALOMYELITIS; CYTOKINE PRODUCTION; MURINE MACROPHAGES; ENDOTOXIN-SHOCK; BETA-CELLS; TNF-ALPHA; NOD MICE;
Keywords:
phosphodiesterase inhibitor; pentoxifylline; rolipram; nitric oxide; macrophage; cytokines; inflammation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Prud'homme, GJ McGill Univ, Dept Pathol, 3775 Univ St,Room B13, Montreal, PQ H3A 2B4, Canada McGill Univ 3775 Univ St,Room B13 Montreal PQ Canada H3A2B4
Citazione:
E. Beshay et al., "The phosphodiesterase inhibitors pentoxifylline and rolipram suppress macrophage activation and nitric oxide production in vitro and in vivo", CLIN IMMUNO, 98(2), 2001, pp. 272-279

Abstract

We studied the effects of the phosphodiesterase inhibitors pentoxifylline (PTX) and rolipram (ROL) on nitric oxide (NO) production by macrophages andcorrelated this with cellular cAMP levels. The RAW 264.7 cell line or mouse peritoneal macrophages were activated with lipopolysaccharide (LPS) and interferon gamma (IFN gamma), with or without ROL, PTX, cAMP analogues, or Forskolin. In vivo peritoneal macrophages were stimulated with staphylococcal enterotoxin B with or without administration of ROL. Nitrite levels in culture and the total cellular cAMP levels were measured. ROL and PTX suppressed NO production of LPS/IFN gamma -stimulated macrophages. ROL (IC50 = 68-74 muM) was about 40 times more potent than PTX (IC50 = 2.4-2..9 mM). The suppression paralleled increased total cellular cAMP level (EC50 = 68-72 muM) and was mimicked by other cAMP elevating agents. ROL and PTX suppressed inducible NO synthase at the mRNA level. The inhibition of NO production of macrophages by ROL or PTX could be beneficial in NO-mediated inflammatory and/or autoimmune disorders. (C) 2000 Academic Press.

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Documento generato il 04/12/20 alle ore 09:31:15