Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Orphenadrine prevents 3-nitropropionic acid-induced neurotoxicity in vitroand in vivo
Autore:
Pubill, D; Verdaguer, E; Canudas, AM; Sureda, FX; Escubedo, E; Camarasa, J; Pallas, M; Camins, A;
Indirizzi:
Nucli Univ Pedralbes, Fac Farm, Unitat Farmacol & Farmacognosia, Barcelona08028, Spain Nucli Univ Pedralbes Barcelona Spain 08028 gnosia, Barcelona08028, Spain Fac Med Ciencias Salut, Unitat Farmacol, Reus 43201, Tarragona, Spain Fac Med Ciencias Salut Reus Tarragona Spain 43201 43201, Tarragona, Spain
Titolo Testata:
BRITISH JOURNAL OF PHARMACOLOGY
fascicolo: 3, volume: 132, anno: 2001,
pagine: 693 - 702
SICI:
0007-1188(200102)132:3<693:OP3ANI>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
NMDA RECEPTOR ANTAGONIST; D-ASPARTATE RECEPTOR; HUNTINGTONS-DISEASE; CEREBRAL-ISCHEMIA; CELL-DEATH; NEURODEGENERATIVE DISEASES; ANIMAL-MODEL; IN-VITRO; RAT; APOPTOSIS;
Keywords:
orphenadrine; HSP27; peripheral benzodiazepine receptor; N-methyl-D-aspartate; microgliosis; neurodegeneration; laser scanning cytometry;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Camins, A Nucli Univ Pedralbes, Fac Farm, Unitat Farmacol & Farmacognosia,Barcelona08028, Spain Nucli Univ Pedralbes Barcelona Spain 08028 rcelona08028, Spain
Citazione:
D. Pubill et al., "Orphenadrine prevents 3-nitropropionic acid-induced neurotoxicity in vitroand in vivo", BR J PHARM, 132(3), 2001, pp. 693-702

Abstract

1 Previous studies indicate that 3-nitropropionic acid (3-NPA) neurotoxicity involves the excitotoxic activation of N-methyl-D-aspartate (NMDA) receptors. Thus, we examined the effect of orphenadrine (an anticholinergic drugwith NMDA receptor antagonist properties) on 3-NPA neurotoxicity in both cultured rat cerebellar granule cells (CGCs) and in rats.2 Orphenadrine protected CGCs from 3-NPA-induced mortality, as assessed byboth the neutral red viability assay and laser scanning cytometry, using propidium iodide staining.3 For rats, two indirect markers of neuronal damage were used: the bindingof [H-3]-PK 11195 to the peripheral-type benzodiazepine receptor (PBR), a microglial marker, and expression of the 27 kD heat-shock protein (HSP27), a marker of activated astroglia. Systemic administration of 3-NPA (30 mg kg(-1) per day for 3 days) induced a 170% increase in [H-3]-PK 11195 binding,and expression of HSP27.4 Both the increase in [H-3]-PK 11195 and HSP 27 expression were preventedby previous administration of 30 mg kg-l per day of orphenadrine for 3 days. Lower doses (10 and 20 mg kg(-1)) had no protective effect. Orphenadrinealso reduced 3-NPA-induced mortality in a dose-dependent manner.5 We propose that orphenadrine or orphenadrine-like drugs could neurodegenerative disorders mediated by overactivation of NMDA receptors.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/20 alle ore 03:40:03